植物法呢基焦磷酸合酶(farnesyl pyrophosphate synthase,FPPS)是甲羟戊酸代谢途径分支点的关键酶,参与叶绿素、类胡萝卜素、细胞分裂素、脱落酸、赤霉素、长萜醇、类萜、辅酶Q、甾醇和植物毒素等类异戊二烯物质的合成,而类异戊二烯是维持植物生长发育等所必需的重要有机物质。本文综述了植物法呢基焦磷酸合酶基因克隆、基因表达、酶动力学研究等方面的重要进展,旨在为其在植物进化分类、遗传改良中的应用提供参考。
Plant farnesyl pyrophosphate synthase(FPPS) is a key branch-point enzyme in mevalonate pathway, and it is involved in the synthesis of isoprenoids viz. chlorophylls, carotenoids, cytokinins, abscisic acid, gibberellins, dolichols, terpenoids, ubiquinones, sterols and phytotoxins required in the essential biological processes such as growth, development, reproduction and adaptation to the environmental challenges. To provide the theory basis of FPPS in plant evolution study and genetic improvement, this paper summarizes the advances in the cloning and expression of FPPS gene, and its enzyme kinetics.