中文摘要：

自2003年DNA条形码（DNAbarcodes）概念出现以来，DNA条形码技术（DNAbarcoding）受到生物分类学领域普遍关注，线粒体细胞色素氧化酶亚基I（mtDNAcoI）被用作动物类群的主要条形码序列，基于该基因片段的昆虫条形码研究在国内外广泛开展。本文在概述DNA条形码、条形码技术及已开展的昆虫条形码研究计划的基础上，总结了昆虫mtDNAcoI条形码及其技术在发现和描述隐种、种类分子鉴定以及系统发育等方面的研究进展，分析了细胞核线粒体假基因（Numts）对mtDNACOI条形码扩增的影响，提出检测和避免Numts的方法，并对DNA条形码技术的进一步研究和应用进行了讨论和展望。

英文摘要：

DNA barcoding has been widely used in taxonomy since the concept appeared in 2003. The mitochondrial gene cytochrome c oxidase I （ COI） is regarded as one of the most acceptable molecular markers and insect DNA barcoding research based on this gene has been developed internationally. This paper gives a general introduction to DNA barcodes, DNA barcoding and insect DNA barcode projects. It summarizes research into and the application of insect mtDNA COI barcoding in the description of cryptic species, species molecular identification and phylogenetics. In addition, we analyse the influence of coamplification of Numts with mtDNA COI barcodes, presenting several suggestions on detecting and avoiding Numts. Finally, we discuss future prospects for DNA barcoding.