中文摘要：

为了进一步探讨四眼斑水龟（Sacalia quadriocellata）的繁殖生理周期和生殖激素分泌特征，使用放射免疫分析测定法（RIA）分别测定了8月（夏季）、10月（秋季）、1月（冬季）、3月（春季）四眼斑水龟血浆中卵泡刺激素（FSH）、促黄体生成素（LH）、睾酮（T）、雌二醇（E2）、孕酮（P）五种生殖激素的含量。结果显示，四眼斑水龟生殖激素分泌呈现较明显的周期性，激素水平与环境温度的变化有关；雄性T含量夏季开始升高，秋季达到高峰，与精子的发生和成熟同步；雌性T水平升高促进其接受雄性爬胯，且作为雌激素合成的前体物质，间接作用于雌激素的合成；排卵会出现LH峰，E2含量排卵前几个月开始增长，刺激肝生成卵黄；排卵期间P含量较高，可能在排卵过程中发挥作用。

英文摘要：

In this paper, the plasma gonadotropins including follicle-stimulating hormone （FSH）, luteinizing hormone （LH）, gonadal steroids testosterone （T）, estradiol （ E2 ） and progesterone （P） of freshly captured Four Eye-spotted Turtle （Sacalia quadriocellata ） were measured using a radio-immunoassay （RIA） method. The result shows that： The reproductive hormones in plasma displayed a distinct seasonal cycles. The plasma gonadal steroids levels are correlation to environmental temperature, as highest during summer and autumn, lowest in winter. Plasma T elevated during the period gonadal maturation and spermatogenesis occur. In female, there was a preovulatory LH surge and T rise during mating period, which probably is related to receptivity and coincided with ovarian maturation. Female E2 levels increased prior to mating season and appeared to stimulate vitellogenin production by liver. A sharp surge of P displayed during mating period （autumn） which coincided with the timing of ovulation.