中文摘要：

SanglifehrinA的产生菌淡黄色链霉菌Streptomyces flaveolus DSM9954，通过筛选，得到其菌丝培养基为TSB，生孢培养基为ISP-4，抗性实验显示淡黄色链霉菌Streptomy—ces flaveolus DSM9954对所选抗生素都较敏感．利用原生质体转化和接合转移都成功地构建了两种遗传转移系统，并且从接合转移成功的链霉菌转化子中回收质粒pKC1139，经EcoRI单酶切电泳，验证了质粒确实通过接合转移从大肠杆菌转移至宿主链霉菌，这为研究SanglifehrinA的生物合成机制和结构改造奠定了基础．

英文摘要：

Sanglifehrin A is produced by Streptomyces flaveolus DSM 9954. TSB culture was selected as its growing culture, ISP-4 as sporing culture. Antibiotic resisting test showed S. flaveolus DSM 9954 was sensitive to all the chosen antibiotics. The genetic transformation system of S. flaveolus DSM 9954 was successfully developed either by the protoplast transformation or conjugation between S. flaveolus and E. coll. And the plasmid pKCl139 was rescued from the transformants and confirmed by EcoR I digestion, which proved the success of conjugation between S. flaveolus and E. coll. These results laid the foundation for studying Sanglifehrin A＇s biosynthesis pathway and producing its analogues.