中文摘要：

目的利用斑马鱼脊髓损伤模型,研究水温改变对脊髓损伤修复的影响,并检测在脊髓修复过程中,损伤处细胞数目以及脑内相关基因表达的变化情况。方法利用手术构建斑马鱼脊髓损伤模型;通过改变斑马鱼生活的水温来观察其脊髓损伤后游动能力的变化情况;用振动切片技术及免疫组织化学观察脊髓损伤处不同时间点细胞数目的改变情况;实时荧光定量PCR法分析脑内相关基因gdnf、nos的表达差异。结果 28℃、30℃、32℃水温均不会导致未手术组与假手术组斑马鱼游动能力的改变（P〉0.05）;32℃水温环境能够使脊髓损伤组斑马鱼比28℃、30℃水温环境的斑马鱼恢复游动的能力明显增强（P〈0.05）。脊髓切片染色实验结果显示,脊髓损伤后损伤处细胞数目明显增多（P〈0.05）。相同时间点,水温为32℃比28℃、30℃细胞数目增多更加明显（P〈0.05）。实时荧光定量实验,gdnf基因在脊髓损伤术后与对照组相比在24 h、7 d、14 d均明显升高（P〈0.05）;nos基因在脊髓损伤术后24 h明显升高（P〈0.05）,7 d时与相同时间点假手术组相比无明显差异（P〉0.05）,而14 d时与相同时间点假手术组相比明显降低（P〈0.05）。结论适当提高水环境温度可促进斑马鱼脊髓损伤后恢复。

英文摘要：

expression at the injury site during the process of recovery. Methods Surgical operation was performed to induce spinal cord injury （SCI） on adult fish. Water at a series of temperature was applied to culture the fish. Swimming ability was adopted to observe the recovery of spinal cord injury following surgery. Vibration sections and immunohistochemistry were performed to observe the cell number post SCI at different stages. The changes of gdnf and nos gene expression were determined by real-time PCR. Results The water temperature changes from 28℃ to 32℃ did not affect the swimming ability of non-injured and sham-injured fish （P〉0.05）. The swimming ability recovered mostly in 8 weeks post spinal cord injury. At 32℃, the swimming ability recovered faster than at 28℃ or at 30℃ （P〈0.05）. The cell proliferation increased obviously following spinal cord injury （P〈0.05）. The proliferation of cells surrounding the spinal cord in jury was more extensive in SCI fishes incubated in 32℃ water than in 28℃ or 30℃ water （P〈0.05）. Real-time PCR assay showed that gdnf was up-regulated in all groups post SCI at 24 h, and 7 and 14 days （P〈0.05）. The nos expression was up-regulated in all groups following SCI in 24 h （P〈0.05） and 7 days. There was no significant difference between the SCI group and sham-injury group （P〈0.05）, while after 14 days, the expression of nos was reduced in the SCI group compared with the sham-injury group （P〈0.05）. Conclusions A slight increase of incubating water temperature can accelerate the recovery of spinal cord injury in zebrafish.