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Rho激酶在缺氧性肺动脉平滑肌细胞增殖中的作用
  • ISSN号:2095-4352
  • 期刊名称:《中华危重病急救医学》
  • 时间:0
  • 分类:R543.2[医药卫生—心血管疾病;医药卫生—临床医学;医药卫生—内科学] R363[医药卫生—病理学;医药卫生—基础医学]
  • 作者机构:[1]第四军医大学病理生理学教研室,陕西西安710033
  • 相关基金:国家自然科学基金资助项目(30370624)
中文摘要:

目的 研究低氧条件下Rho激酶是否参与低氧引起的血管平滑肌细胞增生。方法 组织块法培养大鼠肺动脉平滑肌细胞(PASMCs)。应用甲基噻唑基四唑(MTT)比色法观察细胞增生情况;流式细胞仪观察细胞周期的变化;蛋白质免疫印迹法(Western blot)检测Rho激酶的表达。结果 缺氧组MTT比色吸光度(A)值显著高于常氧组.缺氧24h+Rho激酶抑制剂Y27632组A值显著低于缺氧24h组;缺氧组细胞周期G2/M期细胞比例显著高于常氧24h组,缺氧24h+Y27632组细胞比例显著低于缺氧24h组。Western blot结果表明各缺氧组Rho激酶表达均明显高于常氧组,其中以缺氧24h组表达增高最明显;Rho激酶在缺氧24h+Y27632组明显低于缺氧24h组。结论 缺氧可诱导PASMCs增殖,缺氧诱导的增生PASMCs中Rho激酶活性水平增加,提示缺氧致PASMCs增殖过程中Rho激酶可能发挥重要作用。

英文摘要:

Objective To study whether Rho kinase is involved in the proliferation of pulmonary arterial smooth muscle cells (PASMCs) induced by hypoxia. Methods Rat's PASMCs were isolated and cultured. Methyl thiazolyl tetrazolium (MTT) was used to determine the growth of cells. The cell cycle was analyzed by flow cytometry. Western blot was used to assess the expression of Rho kinase. Results The results of MTT showed that the number of PASMCs was increased after exposure to low oxygen tension for 12 hours compared with PASMCs in normoxia. After exposure of PASMCs to hypoxia for 24 hours, the cellular proliferation peaked, and was higher than that of PASMCs exposed to normal oxygen tension, hypoxia and Y27632 (Rho kinase inhibitor) groups, then it declined when exposed for 48 hours. Analysis of cell cycle indicated that the ability of cell proliferation increased significantly in PASMCs exposed to hypoxia compared with normoxia. Rho kinase level was higher in PASMCs exposed to hypoxia compared with normoxia. Conclusion Hypoxia stimulates proliferation of PASMCs and promotes PASMCs into the phase of mitosis. The expression of Rho kinase is increased in PASMCs that are exposed to hypoxia, and it shows that Rho kinase is activated by hypoxia in PASMCs. Rho kinase maybe involve in the proliferation of PASMCs induced by hypoxia.

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期刊信息
  • 《中华危重病急救医学》
  • 中国科技核心期刊
  • 主管单位:中华人民共和国卫生部
  • 主办单位:中华医学会 天津市天和医院
  • 主编:
  • 地址:天津市和平区睦南道122号
  • 邮编:300050
  • 邮箱:CCCM@em120.com
  • 电话:022-23042150 23306917
  • 国际标准刊号:ISSN:2095-4352
  • 国内统一刊号:ISSN:12-1430/R
  • 邮发代号:6-58
  • 获奖情况:
  • 国内外数据库收录:
  • 美国化学文摘(网络版),荷兰文摘与引文数据库,美国生物医学检索系统,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版)
  • 被引量:4286