中文摘要：

为探讨入侵植物角果毛茛（Ceratocephalus testiculatus）种子的萌发特性,以温度、赤霉素（GA3）、硝酸钾（KNO3）和浓硫酸腐蚀时间（SACT）等为参试因素的正交试验来研究其种子萌发响应特性。预试验结果表明：在自然条件下角果毛茛种子萌发耗时长,萌发率低,处理14d后才开始有种子萌发,28d后的最终萌发率仅为10%左右,存在种子休眠机制。正交试验研究结果表明,4个参试因素均能极显著地促进该种子萌发（P〈0.01）,处理7d后种子陆续萌发,萌发处理14d时发芽势可达68.89%,萌发处理28d时最高发芽率可达83.33%,成功解除了角果毛茛的种子休眠。各参试因素对角果毛茛种子萌发率和发芽势的影响顺序相同,均为温度〉SACT〉KNO3〉GA3。最有利于种子萌发率的因素水平组合为A3B4D4E5,即萌发温度为10℃,赤霉素浓度为150mg·L^-1,硝酸钾浓度为1.50g·L^-1,浓硫酸腐蚀时间为10min;发芽势的最优水平组合为A3B3D2E4,即萌发温度为10℃,赤霉素浓度为100mg·L^-1,硝酸钾浓度为0.50g·L^-1,浓硫酸腐蚀时间为8min。

英文摘要：

To investigate the seed germination characteristics of invasive plant bur buttercup（Ceratocephalus testiculatus）,four factors（temperature,gibberellic acid（GA3）,potassium nitrate（KNO3）and concentrated sulfuric acid corrosion times（SACT））was to be used to study the seed germination response basing on the orthogonal testing method.The results showed that the germination of bur buttercup seeds under the natural condition needed more time and the germination rate was low.Seeds began to germinate after 14 days,and the final germination rate was only about 10% after 28 days.Obviously,bur buttercup seeds germination existed dormancy mechanism.The results of orthogonal experiment variance analysis indicated that the four factors highly significantly promoted the seeds germination.Treating seeds began to germinate after 7days,and the germination potential of bur buttercup seeds was 69% after 14 days.The highest germination rate reached 83.3% after 28 days treatment,and the bur buttercup seeds dormancy was successfully released.Each experimental factors affecting the bur buttercup seeds germination rate and germination potential was the same order：temperature 〉SACT〉KNO3〉GA3.The optimal condition for seed germination rate was A3B4D4E5（temperature：10℃,concentration of GA3：150mg·L^-1,concentration of KNO3：1.50g·L-1,and sulfuric acid corrosion time：10min）,and the optimal level of germination potential combination was A3B3D2E4（temperature：10℃,concentration of GA3：100mg·L^-1,concentration of KNO3：0.50g·L^-1,and sulfuric acid corrosion time：8min）.