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海洋病毒在海洋微生物群落及生物地球化学循环中的作用研究进展
  • ISSN号:1000-3096
  • 期刊名称:《海洋科学》
  • 时间:0
  • 分类:S852.659.5[农业科学—基础兽医学;农业科学—兽医学;农业科学—畜牧兽医] U675.12[交通运输工程—船舶及航道工程;交通运输工程—船舶与海洋工程]
  • 作者机构:[1]Guangdong Provincial Key Laboratory of Marine Biology, Shantou University, Shantou 515063, China, [2]Marine Biology Institute, Shantou University, Shantou 515063, China
  • 相关基金:Supported by the National Natural Science Foundation of China (No. 41176103), the Foundation for Distinguished Young Talents in Higher Education of Guangdong, China (FDYT) (No. LYM09079), and the International Cooperation Project of Guangdong Science & Technology Department (No. 201113050300026) We thank Prof. WEI Chiju of Shantou University for his critical discussion and proof-reading of the manuscript. The authors thank Dr. LI Ping and Mr. HUANG Zhongwen for their assistance with the collection of samples.
中文摘要:

为了理解基因差异和人口,在 Shantou 的沿海的水里在 cyanophages 变化,东北华南海,我们把衣壳集会蛋白质基因 g20 用作自然 cyanomyovirus 社区的丰富和发展史的一个标记。沿海的水里的许多总数病毒,不是自养的细菌,和 picophytoplankton 与流动 cytometry 被监视。水文学参数没有<潜水艇class=“ a-plus-plus ”> 3 <啜class=“ a-plus-plus ”>,没有<潜水艇class=“ a-plus-plus ”> 2 <啜class=“ a-plus-plus ”>, NH <潜水艇class=“ a-plus-plus ”> 3 ,可溶的反应的磷,全部的溶解的氮全部的溶解的磷,溶解的氧,化学的氧需求,温度,咸度,和叶绿素集中和微生物引起的丰富总数病毒全部的细菌 Prochlorococcus Synechococcus 和优核质被测量在上面一在直接病毒的计数, cyanomyoviruses 占了 1.92% 到 >10% 全部的病毒的社区。种系发生的分析证明 Shantou 沿海的水里的 g20 序列是很多样的,以八个不同运作的分类单位分布式,包括最新形成的簇 W。从实时 PCR 试金推断的 g20 基因拷贝显示 cyanomyoviruses 与不是自养的细菌数字和硝酸盐和叶绿素显著地被相关一集中。这些结果建议 cyanomyoviruses 是无所不在的并且是 Shantou 沿海的水里的 virioplankton 的一个丰富的部件。

英文摘要:

To understand the genetic diversity and population changes in cyanophages in the coastal waters of Shantou, northeast South China Sea, we used the capsid assembly protein gene g20 as a marker of the abundance and phylogeny of natural cyanomyovirus communities. The abundance of total viruses, heterotrophic bacteria, and picophytoplankton in the coastal waters was monitored with flow cytometry. Hydrological parameters (NO3^-, NO2^-, NH3, soluble reactive phosphorus, total dissolved nitrogen, total dissolved phosphorus, dissolved oxygen, chemical oxygen demand, temperature, salinity, and chlorophyll a concentration) and microbial abundance (total viruses, total bacteria, Prochlorococcus, Synechococcus, and eukaryotes) were measured in the upper and lower layers at four sampling sites in the research area. In the direct viral counts, cyanomyoviruses accounted for 1.92% to 〉10% of the total viral community. A phylogenetic analysis showed that the g20 sequences in the Shantou coastal waters were very diverse, distributed in eight distinct operational taxonomic units, including the newly formed Cluster W. The g20 gene copies inferred from real time PCR assay indicated that cyanomyovimses were correlated significantly with the heterotrophic bacteria numbers and the nitrate and chlorophyll a concentrations. These results suggest that cyanomyoviruses are ubiquitous and are an abundant component of the virioplankton in Shantou coastal waters.

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期刊信息
  • 《海洋科学》
  • 北大核心期刊(2011版)
  • 主管单位:中国科学院
  • 主办单位:中国科学院海洋研究所
  • 主编:侯一筠
  • 地址:青岛南海路7号
  • 邮编:266071
  • 邮箱:dehail@ms.qdio.ac.cn
  • 电话:0532-82898751
  • 国际标准刊号:ISSN:1000-3096
  • 国内统一刊号:ISSN:37-1151/P
  • 邮发代号:2-655
  • 获奖情况:
  • 中国自然科学核心期刊
  • 国内外数据库收录:
  • 美国化学文摘(网络版),美国剑桥科学文摘,英国动物学记录,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),中国北大核心期刊(2000版)
  • 被引量:21338