为了理解基因差异和人口,在 Shantou 的沿海的水里在 cyanophages 变化,东北华南海,我们把衣壳集会蛋白质基因 g20 用作自然 cyanomyovirus 社区的丰富和发展史的一个标记。沿海的水里的许多总数病毒,不是自养的细菌,和 picophytoplankton 与流动 cytometry 被监视。水文学参数没有<潜水艇class=“ a-plus-plus ”> 3 <啜class=“ a-plus-plus ”>,没有<潜水艇class=“ a-plus-plus ”> 2 <啜class=“ a-plus-plus ”>, NH <潜水艇class=“ a-plus-plus ”> 3 ,可溶的反应的磷,全部的溶解的氮全部的溶解的磷,溶解的氧,化学的氧需求,温度,咸度,和叶绿素集中和微生物引起的丰富总数病毒全部的细菌 Prochlorococcus Synechococcus 和优核质被测量在上面一在直接病毒的计数, cyanomyoviruses 占了 1.92% 到 >10% 全部的病毒的社区。种系发生的分析证明 Shantou 沿海的水里的 g20 序列是很多样的,以八个不同运作的分类单位分布式,包括最新形成的簇 W。从实时 PCR 试金推断的 g20 基因拷贝显示 cyanomyoviruses 与不是自养的细菌数字和硝酸盐和叶绿素显著地被相关一集中。这些结果建议 cyanomyoviruses 是无所不在的并且是 Shantou 沿海的水里的 virioplankton 的一个丰富的部件。
To understand the genetic diversity and population changes in cyanophages in the coastal waters of Shantou, northeast South China Sea, we used the capsid assembly protein gene g20 as a marker of the abundance and phylogeny of natural cyanomyovirus communities. The abundance of total viruses, heterotrophic bacteria, and picophytoplankton in the coastal waters was monitored with flow cytometry. Hydrological parameters (NO3^-, NO2^-, NH3, soluble reactive phosphorus, total dissolved nitrogen, total dissolved phosphorus, dissolved oxygen, chemical oxygen demand, temperature, salinity, and chlorophyll a concentration) and microbial abundance (total viruses, total bacteria, Prochlorococcus, Synechococcus, and eukaryotes) were measured in the upper and lower layers at four sampling sites in the research area. In the direct viral counts, cyanomyoviruses accounted for 1.92% to 〉10% of the total viral community. A phylogenetic analysis showed that the g20 sequences in the Shantou coastal waters were very diverse, distributed in eight distinct operational taxonomic units, including the newly formed Cluster W. The g20 gene copies inferred from real time PCR assay indicated that cyanomyovimses were correlated significantly with the heterotrophic bacteria numbers and the nitrate and chlorophyll a concentrations. These results suggest that cyanomyoviruses are ubiquitous and are an abundant component of the virioplankton in Shantou coastal waters.