中文摘要：

采用在草间钻头蛛Hylyphantes graminicola的饮水和人工饲料中添加四环素的方法研究了蜘蛛体内沃尔巴克氏体（Wolbachia）的去除。同时,通过设置草间钻头蛛Wolbachia感染种群与未感染种群之间不同的交配方式,研究Wolbachia感染对草间钻头蛛生殖和适合度的影响。结果显示,在草间钻头蛛的饮水和人工饲料中添加四环素均能部分去除草间钻头蛛体内的Wolbachia,在供试最高浓度下,去除率分别为40%和50%。4种不同的交配组合下,草间钻头蛛雌蛛均能产卵,卵的孵化率差异不显著,Wolbachia感染对草间钻头蛛的生殖无影响。感染Wolbachia的草间钻头蛛所产第1卵袋的卵历期为6.65d,极显著高于未感染Wolbachia的卵历期（P=0.008）。感染与未感染Wolbachia的草间钻头蛛种群世代存活率、性比以及适合度均无显著差异。

英文摘要：

Through adding tetracycline in drinking water and artificial diet of the spider, the removal methods of Wolbachia in Hylyphantes graminicola were detected. At the same time, different mating ways were conducted between Wolbachia positive population and Wolbachia negative population of H. gramini- cola, and the influences of Wolbachia on the reproduction and fitness of H. graminicola were detected. The result shows that two means can partly remove Wolbachia from H. graminicola, and the maximal re- moval ratio is 40% and 50% respectively. Female from different types of mating pairs in H. graminicola all can produce valid egg sacs, and there is no significant difference on the hatchability of eggs between infected and uninfected spiders. This result illuminates that there is no reproductive influence of Wolba- chia infection on H. graminicola. The duration of eggs （6.65 d） from the first egg sacs of Wolbachia pos- itive H. graminicola is significantly longer than the duration of Wolbachia negative H. graminicola （P = 0. 008 ）. No significant difference is found on the generational survival rate, sex ratio and fitness between Wolbachia positive and Wolbachia negative population of H. graminicola.