中文摘要：

【目的】研究中红侧沟茧蜂Microplitis mediator非典型气味受体的表达谱。【方法】使用RT-PCR法克隆得到中红侧沟茧蜂非典型气味受体基因序列。使用实时荧光定量PCR法研究其表达谱。【结果】克隆得到中红侧沟茧蜂非典型气味受体全长基因。该基因在触角中特异性表达。在雌虫触角中羽化当天达到最大表达量,雄虫触角中在羽化前d阶段达到最大表达量。【结论】该非典型气味受体在中红侧沟茧蜂触角中特异存在。

英文摘要：

[ Objective] The expression of an olfactory receptor of Microplitis mediator was studied. [ Method] The olfactory receptor gene of Microplitis mediator was cloned using RT-PCR. And its tissue-specific expression and quantitative changes at different developmental stages were detected using real-time PCR. [Result] A gene encoding a novel olfactory receptor, which is antenna-specific, was isolated from Microplitis mediator （Hymenoptera： Braconidae）. Its expression levels reached the maximum amount on the emergence day of female and reached the maximum amount at the d stage before emergence of male. [ Conclusion ] This receptor is antenna- specific in Microplitis mediator.