中文摘要：

建立了一种高效、快速的毛细管电泳分离-激光诱导荧光（CE-LIF）检测卡托普利（CAP）和N-乙酰-L-半胱氨酸（NAC）的分析方法。采用1,3,5,7-四甲基-8-溴甲基-二氟二硼-二吡咯甲川（TMMB-Br）为柱前衍生试剂,在50 mmol/L磷酸盐缓冲溶液（pH=8.5）中,40℃下进行衍生反应10min。以荧光素为内标,25mmol/L硼酸盐缓冲溶液（pH=9.5）为电泳背景电解质,14min内达到基线分离。CAP和NAC的检出限分别为0.65nmol/L、0.76nmol/L。将该方法应用于人体尿液和血清中这两种物质的测定,回收率在97.0%~105.7%之间。

英文摘要：

A highly efficient and fast analysis method was developed for the determination of captopril（CAP）and N-acetylcysteine（NAC）by capillary electrophoresis with laser-induced fluorescence detection（CE-LIF）.1,3,5,7-tetramethyl-8-bromomethyl-difluoroboradiaza-s-indacene（TMMB-Br）was used for pre-column derivatization.Fluorescein was used as an internal standard（I.S.）.The optimum derivative reaction was conducted in 50mmol/L phosphate buffer solution（pH=8.5）at 40℃for 10 min.At room temperature,derivatives of captopril and N-acetylcysteine were baseline separated within 14 min using 25mmol/L boric acid solution（pH=9.5）as the running buffer.The detection limits（S/N=3）of the thiol derivatives were 0.65nmol/L and 0.76nmol/L,respectively.The proposed method was tested by analyzing the drugs in human plasma and urine samples with recoveries of 97.0%-105.7%.