中文摘要：

背景与目的姜黄素是从姜黄科植物的根茎中提取的一种天然化合物。体内外的临床前研究表明,其具有抗炎、抗氧化、抗肿瘤等多种作用。miR-186＊是通过microarray芯片技术发现的在人肺腺癌细胞A549/DDP细胞中高表达的基因。本研究旨在阐明姜黄素是否可通过调控miR-186＊的表达而促进A549/DDP细胞的凋亡。方法mi-croarray芯片技术检测经姜黄素、DMSO分别处理后A549/DDP细胞中microRNAs（miRNAs）表达量的变化;实时定量PCR验证从microarray芯片中筛选出来的明显差异表达的miRNAs;最后,流式细胞仪法检测明显差异表达的miR-NAs调控的细胞凋亡,MTT法检测细胞存活率。结果microarray芯片技术结果显示：与DMSO对照组比较,姜黄素处理后miR-186＊的表达明显下调。实时定量PCR验证了microarray芯片技术检测的结果。流式细胞仪法检测miR-186＊调控的细胞凋亡,MTT法检测细胞存活率：姜黄素处理后,与对照组比较,miR-186＊的表达明显下调,从而加速A549/DDP细胞的凋亡,细胞存活率下降;转染miR-186＊的mimetics和其对照后,与对照组比较,miR-186＊的表达上调,从而减少A549/DDP细胞的凋亡,细胞存活率增加。结论姜黄素调控miRNAs的表达可能是其促肺癌细胞凋亡的重要机制之一。

英文摘要：

Background and objective Curcumin,a natural compound,is derived from the rthizom of Curcuma longa.In vitro and in vivo preclinical studies have shown its anti-inflammatory,antioxidant,anticancer activities and so on.miR-186＊,which was found by microarray technology,was highly expressed in lung carcinoma cells A549/DDP.The aim of this study is to illustrate whether Curcumin could promote the apoptosis of A549/DDP cells through regulating the expression of miR-186＊.Methods An oligonucleotide microarray chip was used to profile microRNA （miRNA） expressions in A549/DDP cells treated with and without Curcumin.The significantly differentially expressed miRNA,which was selected from microarray chip,validated by quantitative real-time PCR.Ultimately,the remarkably expressed miRNA modulated the apoptosis assaying by flow cytometry expriments and the survival rate was measured by MTT method.Results The microarray chip results demonstrated： Curcumin altered the expression level of miRNAs compared with untreated control in A549/DDP cell line,miR-186＊ was significantly down-regulated after Curcumin treatment,which confirmed by quantitative real-time PCR.Downregulation of miR-186＊ expression by curcumin elevated the apoptosis,and the survival rate of A549/DDP cells decreased;but up-regulation of miR-186＊ expression by transfection its mimics restrained the apoptosis,the survival rate of A549/DDP cells increased,which were assayed by flow cytometry expriments and MTT method.Conclusion Modulation of miRNAs expression may be an important mechanism underlying the biological roles of Curcumin.