中文摘要：

本文研究氧化石墨烯的合成方法及其在生物传感器中的应用。通过Hummer法氧化天然石墨粉制得氧化石墨,在蒸馏水中利用超声分散将氧化石墨剥片,从而合成了氧化石墨烯（GO）。通过透射电镜图表征了氧化石墨烯的形貌并通过红外光谱证实氧化石墨烯的形成。将所合成的氧化石墨烯与三角形貌的金纳米颗粒（prism AuNPs）、辣根过氧化酶（HRP）和壳聚糖（CHIT）混合修饰到玻碳电极上制得过氧化氢传感器。考察了生物传感器的电化学特性,同时也探讨了测试底液、pH值以及工作电位等实验条件对传感器性能的影响。实验结果表明,该传感器响应迅速,灵敏度高,具有良好的重现性和稳定性。

英文摘要：

This article studied the synthesis method of graphene oxide and the applications of graphene oxide in biosensors.Natural graphite powder was oxide by Hummer method to obtain oxide graphite.Graphene oxide（GO） was synthesized using ultrasonic dispersion to strip graphite oxide films in distilled water.The morphology of graphene oxide was characterized by infrared spectroscopy and transmission electron microscope.A hydrogen peroxide sensor was prepared by modifying glassy carbon electrode with the synthesized graphene oxide,chitosan（CHIT）,prism Au nonaparticles（prism AuNPs） and horseradish peroxidase（HRP）.The influence of the experimental conditions such as pH and applied potential on the electrochemical behavior of biosensor was studied.Experimental results show that the sensor is of quick response,high sensitivity,good reproducibility and stability.