中文摘要：

通过设定不同的氮（N）、磷（P）浓度一次性培养东海原甲藻,研究了该藻的生长状况及不同生长阶段单位细胞DNA,RNA及蛋白质含量的变化,结果表明,不同氮磷浓度对东海原甲藻的生长产生显著影响,最终生物量与初始的氮磷浓度呈正相关,不同培养组的最大生长率存在显著差异（P〈0.05）;DNA含量与培养液中氮浓度相关性极显著（P〈0.01）,但与磷浓度相关性不显著（P〉0.05）;RNA含量与氮磷浓度均呈极显著相关（P〈0.01）,而且在不同的生长阶段其含量也会发生变化,随着培养时间的延长,各培养组的RNA含量显著下降（P〈0.05）;RNA/DNA比值与氮磷浓度均显著相关（P〈0.05）,而且与生长率呈线性相关关系。培养液中氮浓度显著影响到东海原甲藻单位细胞总蛋白含量,磷缺乏也会使总蛋白含量显著降低。

英文摘要：

Changes in growth,cellular DNA,RNA and total protein content related to nitrogen（N） and phosphorus（P） concentrations were studied in a marine dinoflagellate Prorocentrum donghaiense Lu grown in batch cultures.The results show that the growth of P.donghaiense is significantly affected by N and P concentrations.The ultimate biomass depended on the initial N and P concentrations.The maximum growth varied distinctly under different N or P treatments（P0.05）.DNA content had significant correlation with N concentrations in the cultures but not with P.RNA content was strongly associated with both N and P concentrations（P0.01）.Different growth stages could also change cellular RNA content.The RNA content decreased along with the extension of experimental time.RNA/DNA ratio had significant correlation with the N and P concentrations.There appeared to be a linear relationship between the growth rate and the ratio of RNA/DNA.Cellular total protein content was significantly affected by N concentrations.P deficiency could also reduce cellular protein content.