用32个微卫星标记分析了30尾(15尾♀;15尾♂)性成熟(12龄)施氏鲟(Acipenser schrenckii)雌雄个体的遗传差异。结果表明:雌、雄群体的遗传多样性差异没有达到显著水平。在6个微卫星标记中有9个等位基因在雌、雄群体中分布比例差异达到50%以上,其中雌性群体HLJSX 194-239bp、HLJSX201-207bp、HLJSX209-210bp、HLJSX210-178bp和HLJSX215-221bp 5个等位基因频率显著高于雄性群体;雄性群体HLJSX194-184bp、HLJSX201-214bp、HLJSX210-233bp和HLJSX226-194bp 4个等位基因频率显著高于雌性群体,初步表明这些基因位点可能与性别决定位点存在一定连锁关系。本研究结果为施氏鲟早期性别鉴定积累了基础数据。
In this paper, 33 microsatellite markers were used to screen the sex-related DNA markers through analyzing DNA differ-ences between female and male in 12 years old mature Amur sturgeon (Acipenser schrenckii) ( 15 samples), The results showed that there were no significant differences between the female and the male populations. However, 9 alleles derived from 6 SSR loci showed different distributions between female and male groups at a rate of 〉50%. Five alleles HLJSX194-239bp, HLJSX201-207bp, HLJSX 209-210bp, HLJSX210 178bp, and HLJSX215-221 bp were found to be significantly higher frequency in the female samples than those in the male samples. However, there was significantly higher frequency in 4 alleles HLJSX194-184bp, HLJSX201-214bp, HLJSX210-233bp, and HLJSX226-194bp in the male samples than those in the female samples, indicating that these loci are linked with sex-determinate loci. The findings provide a basis for gender identification during early development of Amur sturgeon.