中文摘要：

为了深入研究调控芥菜开花信号整合子的两个核心转录因子Flowering Locus C（FLC）和SHOTR VEGETATIVE PHASE（SVP）的作用机理和进行人工调控,以芥菜‘QJ’为材料,采用RT-PCR技术分别扩增FLC和SVP的编码序列,构建原核表达质粒pET43.1a-FLC、pET43.1a-SVP,转化宿主菌大肠杆菌BL21,通过SDS-PAGE检测该蛋白的表达。利用免疫共沉淀原理及pET43.1a-FLC、pET43.1a-SVP融合蛋白序列中的6×His标签能与Ni＋结合的特点,对芥菜FLC与SVP的相互作用进行SDS-PAGE检测。结果表明：FLC与SVP能在体外相互作用并形成复合体,为深入分析FLC与SVP间的作用机理以及探讨其与下游开花信号整合子元件的相互作用提供了理论和技术基础。

英文摘要：

The fate of the flowering signal integrators is determined by Flowering Locus C（FLC） and SHOTR VEGETATIVE PHASE（SVP）,which probably directly interact with each other to regulate flowering.For further study on mechanism and reagent control of the interactions between the two transcription factors,the protein interactions in vitro were testified in Brassica juncea Coss.（mustard） variety‘QJ’.In the present study,the coding sequences of FLC and SVP were amplified,and then inserted into the expression vector pET43.1a to construct the recombinant plasmids pET43.1a-FLC and pET43.1a-SVP.After transformation to E.coli（BL21）,the expression of recombinant proteins were detected via SDS-PAGE.With co-immunoprecipitation and characteristic of 6 × His tag in fusion protein of pET43.1a-FLC and pET43.1a-SVP which could combine with Ni＋,a new method was put forward to detecting the interactions between FLC and SVP proteins.Using this method,the interactions were analyzed via SDS-PAGE.The results showed that FLC and SVP could act with each other to combine and form a complex.This research provides theoretical and technical bases for analyzing the mechanism of interactions between FLC and SVP,for further probing into interactions between FLC,SVP and downstream targets of flowering signal integrators.