中文摘要：

目的：构建及鉴定头颈部黏膜恶性黑色素瘤（mucosal melanoma of head and neck,HNMM）病人源性肿瘤细胞（patient derived tumor cell,PDC）模型。方法 ：通过肿瘤组织块原代培养、分离肿瘤细胞,利用免疫荧光染色鉴定分离的肿瘤细胞;以PDC皮下成瘤,通过组织切片染色和免疫组织化学染色对比病人原发灶和PDC成瘤组织的细胞学形态。结果：头颈黏膜恶性黑色素瘤患者移植瘤组织块分离出的细胞细胞膜和细胞质HMB-45和Melan-A免疫荧光染色呈阳性。PDC可以在裸鼠皮下成瘤,成瘤组织与患者组织细胞形态一致。结论：头颈部黏膜恶性黑色素瘤构建的PDC模型能够反映患者的组织学特征,PDC模型能够为头颈部黏膜恶性黑色素瘤的研究提供可靠的模型。

英文摘要：

PURPOSE： To establish and validate patient derived tumor cell （PDC） model from mucosal melanoma of head and neck （HNMM）. METHODS： Tumor cells derived from xenografted tumor tissue of patients with mueosal melanoma of head and neck were validated by immunofluorescence staining. Patient derived tumor cells were injected subcutaneously into nude mice. Tumor tissues originated from PDC were compared to tissue from primary tumors by H-E and immunochemistry staining. RESULTS： Immunofluorescence signal of HMB-45 and Melan-A expressed on membrane and cytoplasm of patient derived tumor cell. Patient derived tumor cell could form subcutaneous tumor in nude mice. The histology was similar between subcutaneous tumor and primary tumor under H-E and immunoehemistry staining. CONCLUSIONS： Patient derived tumor cell model can be established from mucosal melanoma of head and neck and be used as a validated preclinical model for drug screening.