为阐明辐射诱发的叶艺兰植株与未经诱变植株的差异,以经辐射诱发的叶艺兰植株(TRIR-2)与未经诱变的全绿植株(TRIR-1)为试材,运用显微镜观察植物叶片的气孔及根尖细胞的染色体并使用Photoshop软件对染色体进行分析。结果表明,(1)TRIR-2的气孔数量、面积、密度值均小于TRIR-1;(2)TRIR-1植株的染色体核型公式为2n=2x=10m+24sm+6st,属较为对称的“2B”型;TRIR-2植株的染色体核型公式为2n=2x=10m+28sm+2st,属不对称的“2C”型。说明辐射诱变产生的叶艺兰突变体气孔特征和染色体结构发生了变化。
In order to expound the differences of Ye-yi orchid that was induced by irradiation and that was not induced, the leaf stomatal and the chromosome of root tip cell were observed by microscope, and the chromosome were further analyzed by Photoshop software. The results showed that, (1) quantity, stomatie size and density of stomata of induced plant (TRIR-2) were less than plant that was not induced (TRIR-1) ; (2) karyotype formulaof TRIR-1 is 2n = 2x = 10m+24sm+6st, a relatively symmetrical 2B type, and karyotype formula of TRIR-2 is 2n =2x= 10m+28sm+2st, an asymmetric 2C type.