Desmodesmus sp.S-1藻株经重离子诱变后得到的-株突变体D90G.19,较其野生型的油脂产率提高了20.6%;同时对野生型和突变株的光合放氧速率,色素组成和叶绿素荧光动力学进行了分析。与野生型相比,突变体D90G-19的光合作用有如下特点:1.D90G.19的光饱和点为500gmol/(m2·s),无论是在强光还是在弱光下,D90G-19的光合作用效率都显著高于其野生型;2.突变体D90G.19对高温的适应性比野生型强:3.在弱碱性条件下,D90G-19的光合效率的提升较之野生型更为显著。培养D90G-19的最佳光照强度应在250~400μmol/(m2·s)之间,最适温度在25~30℃,培养液pH值则以弱碱性为宜(7〈pH≤8)。叶绿素荧光动力学数据表明,突变体D90G-19的光合作用系统在强光下的损伤程度小于野生型,能够耐受更高的光强胁迫。
After induced by heavy-ion beam, a mutant of Desmodesmus sp., D90G-19 was obtained, which had a lipid productivity 20.6% higher than the wild type. The photosynthetic characteristics of mutant D90G-19 was characterized by analysis of photosynthetic oxygen evolution rate, chlorophyll concentration and chlorophyll fluo- rescence. Compared to the wild type, the D90G-19 had a higher photosynthetic efficiency regardless of high or low light intensity, the D90G-19 had a higher temperature tolerance and the D90G-19 had a more significant increase in photosynthetic efficiency under weak base condition. The optimum light intensity, temperature and pH for D90G-19 were 250-400 μmol/(m2-s), 25-30℃ and 7〈pH≤8 respectively. The photo saturation point of D90G-19 was about 500 μmol/(m2.s), and the Y(NO) suggested that the damage of photosynthesis system II in D90G-19 was less than that in wild type when they were exposed to high intensity light.