中文摘要：

植物病原菌侵染寄主的过程就是病原菌和寄主植物相互作用的过程。在这个相互作用过程中,Ⅲ型分泌系统和Ⅲ效应物与病原菌致病密切相关。大部分革兰氏阴性植物病原菌通过Ⅲ型分泌系统定向的把效应物蛋白传递到宿主细胞,效应物蛋白进入植物体引起致病或过敏反应。本研究将百日咳杆菌的腺苷酸环化酶基因连接至含启动子的pLAFRJ载体上,从而构建出一个新的体外快速鉴定Ⅲ型效应物的报告质粒pJ-JA,并用已鉴定为Ⅲ型效应物基因的十字花科黑腐病菌XC1553的启动子和信号区验证该报告质粒,证明这个系统是可以工作的。该报告质粒为进一步精确筛选鉴定十字花科黑腐病菌Ⅲ型效应物提供了材料。

英文摘要：

The infection process of plant pathogen is a interaction between phytopathogens and host plants.Type Ⅲ secretion system and type Ⅲ effectors are closely related and pathogen virulence.Most gram-negative phytopathogens deliver the effector protein directly into the host cell through type Ⅲ secretion system,causing disease or hypersensitive reaction.In this research the adenylate cyclase gene of Bordetella pertussis was cloned into pLAFRJ which contains a promoter,yielding a new reporter plasmid pJJA which can be used to identify the type Ⅲ effectors efficiently in vitro.The promoter and signal region of XC1553 which has been identified as type Ⅲ effectors in Xanthomonas campestris pv.campestris（Xcc） was used to verify the report plasmid.The results of cAMP concentration detection assay in Brassica oleracea leaves demonstrated that the new report plasmid pJJA works well.The report plasmid provides material for further precise screening type Ⅲ effectors in Xcc.