为开发碧桃转录组微卫星信息,利用454高通量测序技术,对其花瓣转录组序列进行SSR位点发掘,结果发现含SSR的序列4705条,共得到5668个SSR,平均每3.49 kb出现1个SSR。微卫星序列主要以三碱基重复为主,约占总数的42.66%。笔者共发现516种碱基重复基元,所占比例最高的为(AG/CT)n(18.34%),其次是( AAG/CTT) n(12.42%)。微卫星多为重复长度小于20 bp 的短序列,长度大于20 bp 的微卫星仅占总数的12.13%。研究还发现碧桃花瓣微卫星的频率和长度呈显著负相关( P<0.05),相关系数为-0.246。
To obtain information of Prunus persica cv. duplex, the transcriptome of peach flowers were sequenced by 454 high-throughput sequencing technology. A total of 5 668 SSRs were identified in 4 705 sequences, with an average densi-ty of one SSR per 3.49 kb. Tri-nucleotide repeats were the most abundant, accounted for 42.66% of all SSRs. Among all the 516 SSR motifs, ( AG/CT ) n was the most frequent repeat motif ( 18.34%) , followed by the ( AAG/CTT ) n ( 12.42%) . The microsatellites in length below 20 bp were in maximum proportion, while the microsatellites over 20 bp were only 12.13%. There were significant negative correlation ( P〈0.05) between the frequency of microsatellites and the length, the correlation coefficient was -0.246.