中文摘要：

为明确一株云烟87八倍体（2n=8x=96）与N.plumbaginifolia Viv.（2n=2x=20）的杂交后代的部分生物学特性。对该后代进行了形态观测,细胞学遗传学分析,育性分析,并进行黑胫病抗性的离体鉴定。结果显示,该杂交后代（无性系）大部分外观形态指数介于云烟87四倍体与N.plumbaginifolia之间;花冠颜色与云烟87相近,花药颜色与N.plumbaginifolia相近。其染色体数目为58条,其中含有来自N.plumbaginifolia的10条染色体。花粉离体培养未见萌发;与云烟87四倍体杂交,做母本时坐果率为100%,平均每个蒴果获得102.60±25.12粒可萌发的种子,以其为父本及自交时未获得成熟蒴果。该杂交后代（无性系）对黑胫病抗性强于云烟87,与N.plumbaginifolia相近。综上所述,该杂交后代为云烟87与N.plumbaginifolia的真杂种（2n=5x=58）;其雄性败育,但雌性可育;且具较强的黑胫病抗性。

英文摘要：

Plant morphology of an offspring between octoploid plant of N.tabacum Lin.cv.Yunyan87（2n=8x=96） and N.plumbaginifolia Viv.（2n=2x=20） was observed.Its cytogenetic characteristics,fertility and its resistance to black shank were investigated.Results showed that most main morphology indexes were between Yunyan87 tetraploid plant and N.plumbaginifolia.Corolla color was similar to Yunyan87 while anther color was similar to N.plumbaginifolia.It had 58 chromosomes,among which 10 were from N.plumbaginifolia detected by GISH.Pollen did not germinate in vitro and no capsules were harvested when the hybrid（clones） was crossed to Yunyan87 tetraploid plants as male and self.102.60± 25.12 germinating seeds were obtained in every capsule when the hybrid crossed to Yunyan87 tetraploid plants as female.No black shank specks on hybrid leaf and stem were found after P.parasitica infection.This was the same with N.plumbaginifolia.The offspring was the sure hybrid of N.tabacum and N.plumbaginifolia（2n=5x=58）.It was male sterile but female fertile and has strong resistance to black shank etiology.