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中文摘要：
目的以OMP18的B细胞抗原表位多肽为包被抗原,建立检测空肠弯曲菌感染的ELISA方法。方法以不同浓度梯度（0.1,1,10μg/mL）OMP18的B细胞抗原表位多肽进行包被,以空肠弯曲菌全菌兔抗IgG为一抗,HRP标记的羊抗兔抗体IgG为二抗,检测对原浓度1mg/mL的不同稀释度（1∶10,1∶100,1∶1 000）抗体IgG水平。分别以空肠弯曲菌感染兔血清、健康兔血清及沙门菌感染兔血清为一抗,1∶3 000稀释的HRP标记的羊抗兔IgG为二抗,比较各抗原表位多肽的免疫特异性。结果 OMP18的B细胞抗原3个表位多肽在稀释度在1∶1 000、1∶4 000、1∶16 000、1∶64 000时免疫反应性均有明显增高。其中稀释度在1∶1 000时增高最明显。OMP18的B细胞抗原表位多肽具有免疫特异性。结论用OMP18的B细胞抗原表位多肽作为包被抗原对空肠弯曲菌感染的血清进行ELISA检测,免疫反应性高,具有免疫特异性。
英文摘要：
This study aims to establish an ELISA method for detection of Campylobacter.jejuni infection by using the Bcell antigen epitope polypeptide of OMP18 as the coating antigen. Using the B-cell epitope peptides with different concentrations （0.1,1 and 10 μg/mL） as the coating antigens, the rabbit anti-Campylobacter jejuni IgG antibody as the primary antibody and the HRP-labeled goat anti-rabbit IgG antibody as the seeondary antibody, the researchers detect the levels of IgG antibody after different dilutions （1 ; 10, 1 ; 10 and 1 ： 1 000） based on its original concentration of 1 mg/mL. The researchers also compare different immunological specificities of epitope peptides via using the serum from a healthy rabbit, the serums respectively from the rabbits infected with Campylobacterjejuni and Salmonella as the primary antibody as well as the HRP-labeled goat anti-rabbit IgG antibody（1 ： 3 000） as the secondary antibody. The immunoreaetivity of three B cell antigen epitope polypeptides of OMP18 significantly increase in the dilutions of 1 ： 1 000,1 ： 4 000 and 1 ： 16 000 and 1 ; 64 000,while it finds the most significant increase in a 1 ： 1 000 dilution. Therefore, the B-eell epitope peptides of OMP18 indeed features an immunological Specificity. And the study indicates that the ELISA is a desirable method capable of detecting Campylobacter jejuni infection by using OMP18 B cell antigen epitope polypeptide as the coating antigen with a high immune response and a specific immunity.
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