为了进一步阐明环境中抗菌药物在土壤—蔬菜系统中的迁移过程与规律,以磺胺甲恶唑(SMX)为目标抗菌药物,采用水培蔬菜方式,测定根际暴露24 h内上海青、木耳菜和快菜体内SMX含量的变化,对比根际暴露24 h后SMX在植物体不同部位中的浓度分布,计算SMX在蔬菜叶中的累积速率,研究SMX的迁移规律。结果表明,根际分泌物不会影响上海青和快菜根际吸收SMX,但会影响SMX从根部向叶中传输;根际分泌物不仅会促进木耳菜根际吸收SMX,同时也会影响木耳菜根部吸收的SMX向叶中迁移;根际暴露1 h内即可在叶中检出29.6~383.2μg/kg的SMX;3种蔬菜叶中SMX的累积速率普遍随暴露时间增加而降低,且不同蔬菜的累积速率有所差异。植物根际SMX浓度及植物根际分泌物可能影响SMX向植物中的迁移。
The aim of the present study was to clarify the migration processes and rules of environmental antibiotics in the soil-plant system. The sulfamethoxazole (SMX) was used as target antibiotic to characterize its content variation in soilless cultivated pakchoi, Malabar spinach and Chinese cabbage during a 24 h root exposure. The concentration distribution of SMX in different parts of the selected vegetables after 24 h root exposure was statistically assessed and the accumulation rate of SMX in the leave of the vegetables was calculated to evaluate the migration rules of SMX. The results revealed that root exudates showed no effect on absorption of SMX in root of pakchoi and Chinese cabbage, but they showed effect on SMX transmission from root to leave;root exudates not only promoted the absorption of SMX in root of Malabar spinach, but also showed effect on transmission of SMX absorbed from root to leave;29.6-383.2μg/kg of SMX could be determined in the leave of the selected vegetables after 1 h root exposure;the accumulation rate of SMX in the leave of the vegetables was generally reduced with the extension of root exposure time, and it varied in different varieties of vegetables. The combined data suggests that the concentration of SMX in root and root exudates may have influence on the migration of SMX to the plants.