昆虫肠道内存在种类繁多、数量庞大的微生物,这些肠道微生物种群结构的多样性与昆虫种类、龄期、消化道形态、食物的喂养条件、生存环境等息息相关.近几年,随着大规模测序技术、组学技术的发展,应用分子生物学技术快速、定性、定量研究昆虫肠道微生物种群的多样性已成为热点.介绍了昆虫肠道微生物的分类和检测方法,综述了昆虫肠道微生物多样性的研究进展,以期为昆虫与其肠道微生物的协同进化和害虫防治等研究提供基本方法和数据,为今后昆虫肠道微生物的研究提供理论参考.
There are a wide variety and large number of microbes in insect gut. The population diversity of intestinal microbes is related to insect species, age, intestinal morphology, food, feeding conditions and living environment, etc. In recent years, rapid, qualitative and quantitative study of intestinal microbial diversity by molecular biology techniques has become a hot spot with the development of the large-scale sequencing and omics technologies. In this paper, the classification and detection method of the insect intestinal microbes were described, and the progress on intestinal microbial diversity of insects was reviewed. Those would provide basic data and methods for research of insect gut microbes and pest control.