中文摘要：

目的：探究宿主细胞硫氧还蛋白互作蛋白（TXNIP）与 HCV 复制的关系以及 TXNIP 促进HCV 复制的分子机制。方法利用转录组芯片和转录组深度测序技术对细胞培养的 HCV（HCVcc）感染的 Huh7细胞进行转录组分析，并通过反转录-实时荧光定量 PCR 和高通量测序差异表达的 TXNIP 进行细胞差异表达验证；同时观察 TXNIP 表达水平对 HCV 感染的影响。结果转录组芯片检测发现， HCVcc 感染的 Huh7细胞中 TXNIP mRNA 较未感染的对照细胞上调约3．7倍；转录组深度测序表明， TXNIP mRNA 在 HCVcc 感染后36、60 h 均明显上调（t 值分别为24．90、8．27，均 P 〈0．01）；细胞实验证明，小干扰 RNA（siRNA）干扰 TXNIP 表达可降低 HCVcc 感染，而过表达 TXNIP 可促进 HCV 感染；HCV 感染可诱导 Huh7细胞 TXNIP 上调而不能诱导 Huh7．5．1细胞 TXNIP 上调；α干扰素、β干扰素和λ干扰素处理细胞均可上调 TXNIP 的表达。结论 HCV 感染诱导 TXNIP 表达上调，而 TXNIP 表达上调可促进 HCV 复制，宿主蛋白 TXNIP 在 HCV 复制、感染及免疫逃逸中具有重要作用。

英文摘要：

Objective To explore the relationship between host cell thioredoxin-interacting protein （TXNIP）and hepatitis C virus （HCV）replication,as well as the mechanism of how TXNIP promotes HCV replication.Methods The transcriptome shift of Huh7 cells after cell cultrued HCV （HCVcc）infection was analyzed by using Glue Grant Human Transcriptome Microarray.The differential expression of TXNIP was confirmed by reverse transcription-polymerase chain reaction （RT-PCR）and high-throughput sequencing.The effect of TXNIP on HCV infection was tested by TXNIP overexpression and TXNIP knockdown.Results Transcriptome array demonstrated a 3.7 times fold upregulation of TXNIP mRNA in HCVcc infected Huh7 cell higher than those without HCVcc infection,which was further verified by high-throughput deep sequencing.The TXNIP mRNA level was significantly up-regulated at 36 h and 60 h post HCV infection （t=24.90 and 8.27, respectively,both P〈0.01）.In cell level analysis,knockdown of TXNIP by small interfering RNA （siRNA） could reduce HCV infection,while TXNIP overexpression could enhance HCV replication.Infection with HCVcc could increase the expression of TXNIP in Huh7 but not in Huh7.5.1 cells,and interferons α,β,and γtreatment could up-regulate TXNIP expressions in both cell lines.Conclusions HCV infection induces host cell TXNIP expression,and upregulated TXNIP enhances HCV replication.Host cell TXNIP plays an important role in HCV replication,infection and immune escape.