利用外源甲醇(MeOH)处理棉叶后,对棉叶中H_2O_2、·O^-_2两种活性氧含量以及过氧化氢酶(CAT)、过氧化物酶(POD)、超氧化物歧化酶(SOD)3种抗氧化酶活性进行了连续20d的监测。发现经MeOH处理后的棉叶中H_2O_2和·O^-_2含量分别下降了17.80%和22.56%,CAT、POD和SOD活性分别增加53.40%、39.38%和19.59%,监测期内测定项目变化趋势与温度的变化较为一致。表明外源MeOH可抑制光呼吸,减轻H_2O_2造成的光抑制危害。
The two active oxidates H_2O_2,·O^-_2in cotton leaf and antioxidant enzyme(CAT),peroxidase(POD),superoxide dismutase(SOD)were investigated for continuous 20 days.It was found that the content in H_2O_2 and ·O^-_2were decreased by 17.80% and 22.56%,the activity of CAT,POD and SOD were increased by 53.40%,39.38%and 19.59%,respectively,and the result of the tested two active oxidates and activity of the three antioxidant enzyme activities had a close relation with changes of the temperature,which showed that exogenous MeOH can inhibit photorespiration and alleviate the photoinhibition damage.