中文摘要：

目的探讨年轻人正常皮肤、自然老化皮肤、光老化皮肤的弹力纤维形态学特征，建立评判皮肤弹力纤维损伤程度及治疗药物对其修复程度的简单、实用方法。方法分别取年轻人光保护皮肤（年轻人正常皮肤）、老年人光保护皮肤（自然老化皮肤）以及老年人光暴露皮肤（光老化皮肤）进行甲醛溶液固定，石蜡包埋、切片，醛品红染色（Gomori染色法）。结果在年轻人光保护皮肤耐酸纤维、前弹力纤维及成熟的弹力纤维组成结构完整清晰的弹力纤维网络。老年人光保护皮肤的弹力纤维损伤相对较轻，主要表现为真皮乳头处耐酸纤维数量明显减少，真皮中上层弹力纤维轻度增粗及片段化。老年人光暴露皮肤的弹力纤维损伤明显，表现为真皮乳头处耐酸纤维消失，真皮浅层和中层弹力纤维明显增粗、扭曲、断裂、分支、形成团块。结论年轻人正常皮肤、自然老化皮肤、光老化皮肤的弹力纤维形态学各具有明显的特征，Gomori染色法是评价皮肤老化过程中弹力纤维损伤效果及治疗药物对其修复效果的简单、实用方法。

英文摘要：

Objective To investigate the morphological alteration of elastic fibers in normal young skin, intrinsically aged skin and photoaged skin and to set up a simple and low-cost method to evaluate the damage elastic fibers during cutaneous aging and the repair by anti-aging therapy. Method Skin biopsies taken from sun-protective skin of young people （normal young skin）, from sun-protective skin of old people （intrinsically aged skin） and from sun-exposed skin of old people （photoaged skin）, were formalinfixed, paraffin embedded and stained with Gomori method. Results In sun-protective normal skin of young volunteers, oxytalan fibers, elaunin fibers and mature elastic fiber formed a distinct elastic network; in sun-protective normal skin of aged people oxytalan fibers were reduced in papillary dermis, however elastic fibers in the upper and mid-dermis were slightly thickened and fragmented; in sun-exposed human skin of the elderly few disrupted oxytalan fibers were observed in the papillary dermis, and accumulation of dystrophic elastotic material in the upper and reticular dermis. Conclusions Morphological difference of elastic fibers among young normal skin, intrinsically aged skin and photoaged skin is distinct. Gomori staining is a simple and low-cost method to evaluate the damage of elastic fibers during cutaneous aging and to evaluate the repair by anti-aging therapy.