中文摘要：

目的观察糖络宁对糖尿病大鼠背根神经节线粒体DNA、8-羟基脱氧鸟苷（8-OHd G）及线粒体结构的影响。方法选取14只SD大鼠作为正常组,其余SD大鼠采用链脲佐菌素制作糖尿病大鼠模型,造模成功后,将其随机分为模型组、西药组（α-硫辛酸）、中药组（糖络宁）,共给药8周,给药结束后,检测线粒体DNA水平、8-OHd G水平,并运用电镜观察线粒体内部结构。结果与正常组比较,模型组大鼠神经细胞线粒体DNA、8-OHd G水平明显增加（P〈0.01）;与模型组比较,中药组与西药组神经细胞线粒体DNA、8-OHd G水平明显降低（P〈0.01）。与正常组比较,模型组大鼠DRG线粒体肿胀明显,内部结构破坏严重,线粒体内特异结构嵴消失;而中药组大鼠DRG线粒体结构相对完整,线粒体嵴保存完好;西药组大鼠DRG线粒体也存在轻度肿胀,内部结构不完整,嵴显示不清楚。结论糖尿病大鼠神经细胞中线粒体会发生明显改变,其内部结构严重破坏,DNA表达过度升高,DNA的氧化损伤也非常明显。糖络宁可明显减低线粒体DNA表达,减少氧化损伤,缓解线粒体结构的破坏,这是糖络宁治疗糖尿病周围神经病变的机制之一。

英文摘要：

Objective To investigate the effect of Tangluoning on activities of mitochondrial DNA, 8-OHd G and structure of electron microscope in dorsal root ganglion in diabetic rats. Methods Fourteen SD rats were selected as normal group, the other SD rats were given streptozotocin to make diabetic rats model, after success, they were randomly divided into model group, Western medicine group（α-lipoic acid）, TCM group（Tangluoning）. The activities of mitochondrial DNA, 8-OHd G were detected, and the mitochondrial structure was observed by electron microscope after 8 weeks′treatment. Results Compared with normal group, the activities of mitochondrial DNA and 8-OHd G in model group were significantly increased（P〈0.01）. Compared with model group, the activities of mitochondrial DNA and 8-OHd G in Western medicine group and TCM group were significantly decreased（P〈0.01）. Compared with normal group, diabetic rats DRG mitochondria was swelled, the internal structure was damaged obviously and seriously, the specific structure of the mitochondria cristae was disappeared; and DRG mitochondria of TCM group had relatively intact structure, the mitochondrial cristae was well preserved; DRG mitochondria of Western medicine group also existed mild swelling, the internal structure was not complete, crest display was not clear. Conclusion In diabetic rats, the mitochondria of nerve cells is changed significantly, the internal structure is damaged seriously, the DNA expression is increased excessively,and the oxidative damage of DNA is also very obvious. Tangluoning can significantly decrease the expression of mitochondrial DNA, reduce the oxidative damage, and alleviate the destruction of mitochondrial structures, which is one of the mechanisms of Tangluoning in the treatment of diabetic peripheral neuropathy.