中文摘要：

目的：对大鼠肝癌模型行经动脉栓塞治疗,探讨栓塞治疗对肝癌肺转移及E-cadherin表达的影响。方法：选取40只Buffalo大鼠,建立稳定表达绿色荧光蛋白的大鼠肝癌模型。将大鼠随机分成4组,每组10只。对照组（A组）：胃十二指肠动脉逆行插管,注入0.9%氯化钠液0.2 mL;肝动脉结扎组（B组）：分离肝动脉后用缝线结扎;小剂量栓塞组（C组）：胃十二指肠动脉逆行插管,注入超液化碘油（0.2 mL/kg）;大剂量栓塞组（D组）：胃十二指肠动脉逆行插管,注入超液化碘油（0.4mL/kg）。术后观察体质量及肿瘤变化。21 d后处死大鼠,收集肝和肺标本,观察肿瘤生长变化及肺转移情况。采用实时荧光定量–聚合酶链反应法、蛋白质印迹法、免疫组织化学法检测肿瘤组织中的E-cadherin的mRNA及蛋白表达水平。结果：术后21 d,D组肝脏肿瘤生长较其他3组明显受抑;B、C组肝脏肿瘤生长较A组明显受抑（P〈0.01）。D组肺转移个数少于其他3组（P〈0.01）。D组E-cadherin mRNA和蛋白相对表达水平高于A、B、C组,差异均有统计学意义（P〈0.01）。D组Ecadherin阳性细胞百分比高于其他3组,差异均有统计学意义（P〈0.01）。结论：在大鼠肝癌模型中,经动脉栓塞治疗肝癌可增加E-cadherin表达,大剂量的碘油栓塞能抑制肿瘤生长,降低肺转移。

英文摘要：

Objective：To conduct transarterial embolization（TAE）in rat model of hepatic carcinoma and to investigate the effect of embolization on pulmonary metastasis and expression of E-cadherin.Methods：Forty Buffalo rats were chosen and rat models of hepatic carcinoma with stable expression of green fluorescent protein（EGFP）were established.Rats were randomly divided into four groups with 10 rats in each group.The control group（Group A）were treated with 0.2 mL of 0.9% sodium chloride solution by gastroduodenal artery retrograde catheterization.The hepatic artery ligation group（Group B）were treated with suture ligation after separation of hepatic artery.The low dose embolization group（Group C）were treated with lipiodol ultrafluide（0.2 mL/kg）by gastroduodenal artery retrograde catheterization.The high dose embolization group（Group D）were treated with lipiodol fluide（0.4 mL/kg）by gastroduodenal artery retrograde catheterization.Body weights,tumor volumes were evaluated after operation.At 21 d after operation,the rats were killed and the samples of liver and lung were collected.The changes of tumor growth and the situations of pulmonary metastasis were evaluated.The changes in E-cadherin mRNA and protein expression levels were detected by real-time fluorescent quantitative PCR,Western blotting and immunohistochemistry.Results：At 21 d after operation,the hepatic tumor growth was obviously inhibited in Group D,and the tumor growth in Group B or Group C was also inhibited while compared with that in Group A（P〈0.01）.The number of pulmonary metastasis in Group D was lower than those in the other groups（P〈0.01）.The levels of E-cadherin mRNA and protein in Group D were higher than those in the other groups and the differences were statistically significant（P〈0.01）.The rate of E-cadherin-positive cells in Group D were higher than those in the other groups according to immunohistochemistry,and the differences were statistically significant（P〈0.01）.Conclusions：TAE,in the t