中文摘要：

目的：探讨核仁磷酸蛋白（Nucleophosmin／B23）在人结肠癌组织中的表达及对人结肠癌细胞侵袭能力的影响。方法：选取2000年6月至2005年10月就诊于天津医科大学附属肿瘤医院结肠癌患者31例，并收集其肿瘤组织、对应的癌旁组织和转移淋巴结组织，采用免疫组织化学染色方法检测B23的表达情况。采用Westernblotting技术检测不同结肠癌细胞系中B23的表达情况，利用小干扰RNA技术下调B23在结肠癌细胞中的表达，利用Transwell侵袭实验观察B23表达下降对结肠癌细胞侵袭能力的影响。结果：免疫组织化学染色显示B23在结肠癌组织的表达高于结肠癌旁组织表达（P=0．0016），在转移淋巴结中的表达高于结肠癌旁组织（P=0．0007），差异有统计学意义。Westernblottingi证实在转染B23特异性小干扰RNA的结肠癌细胞HCT116中B23的表达明显下降，同时明显抑制结肠癌细胞的侵袭能力。结论：B23在结肠癌和转移淋巴结中高表达，且能影响结肠癌细胞的侵袭能力。提示B23可能在结肠癌的发生、进展和浸润转移中起调节作用。

英文摘要：

Objective： To study the expression of B23 in human colon cancer tissues and its role on invasion in colon cancer cells. Methods： A total of 31 patients with colon cancer, who were admitted to the Tianjin Medical University Cancer Institute and Hospital from 2000 to 2005 were enrolled in the current study. Immunohistochemical assay was used to analyze the expression of B23 in colon cancer, para-neoplastic tissues of the cancer, and lymph nodes tissues. Statistical analysis of the immunohistochemical results was conducted using the SPSS 17.0 software. The expression orB23 in different colon cancer cell lines was determined by Western blot. In addition, the small interfering RNA （ siRNA ） technology was utilized to down-regulate the expression orB23 in the HCT116 cells. The invasive ability of the colon cancer cells was observed after the knock down of B23 via the Transwell assay. Results： Immunohistostaining results revealed that the expression of B23 was higher in colon cancer than in the paraneoplastic tissues. Statistically significant differences between the expression of B23 in the colon cancer and lymph nodes were noted and compared with the paraneoplastic tissues adjacent to the colon cancer. The P values were 0.0016 and 0.0007, respectively. Western blot assay confirmed that the B23-specific siRNA could effectively knock down the expression of B23, and the decrease of the B23 expression notably inhibited the invasive ability of the colon cancer cells. Conclusion： B23 showed high expression in colon cancer and lymph node metastasis. The knockdown of B23 can inhibit the migration capability of the colon cancer cells. B23 may play an important role in the progression and metastasis of colon cancer.