通过考察电泳分析条件对葫芦巴中有效成分分离和检测的影响,建立天然药物胡芦巴中3种组分胡芦巴碱(TR)、槲皮素(QU)和柚皮素(NA)的电泳分析方法.结果表明,毛细管电泳在工作电压为20 kV,电泳介质浓度10 mmol·L-1、pH为7.8的硼砂-KDP体系,曲拉通X-100和DMF(N,N-二甲基甲酰胺)体积分数均为0.25%的条件下,能在7 min内实现对3种有效成分的检测.TR、QU和NA的峰面积和浓度分别呈良好的线性关系,线性范围在3.0~200.0,5.0~150.0和1.0~200.0μg·mL-1;检测限分别为0.7、1.0、0.2μg·mL-1.峰面积和迁移时间的RSD分别为:TR,3.2%和1.3%;QU,4.8%和0.83%;NA,4.3%和1.2%.
A capillary electrophoresis method has been developed for the determination of trigonelline, quercetin and naringenin in fenugreek. The analytical conditions were optimized after investigating applied voltage, the effects of buffer pH and concentration, and additives concentration. The 3 analytes were completely separated within 7 min using 10 mmol · L-1 KDP - borax (pH 7.8 ) containing 0.25% (V/V) triton X - 100 and 0.25% (V/V) DMF as additives with applied voltage 20 kV. A good linear relationship between peak area and concentration was observed in the range of 3.0 - 200.0, 5.0 - 150.0, 1.0 - 200.0 μg · mL-1 for TR, QU and NA, respectively, whereas the detection limits based on signal levels three times of noise levels were found to be 0.7, 1.0, 0.1 μg · mL-1 for these three analytes, respectively. The repeatability based on peak area and migration times was 3.2% and 1.3% for TR, 4.8% and 0.83% for QU, 4.3% and 1.2% for NA.