中文摘要：

目的：探讨肾细胞癌患者肾癌组织及其相应癌旁（〉5cm）组织中胰岛素样生长因子Ⅱ（IGF-Ⅱ）基因的印迹状态,初步研究其在肾细胞癌的发生、发展中的关系.方法：根据IGF-Ⅱ基因第9外显子具有Apal位点多态性,用聚合酶链反应结合限制性片段长度多态性技术分析IGF-Ⅱ基因印迹状态.结果：30例肾细胞癌患者中癌及相应癌旁组织IGF-Ⅱ均发生杂合子基因型的有19例（63.3%）,其中癌及相应癌旁组织均发生IGF-Ⅱ印迹丢失（LOI）13例（68.4%）;癌组织发生IGF-ⅡLOI而其相应的癌旁组织未发生IGF-ⅡLOI为1例（5.3%）;22例透明细胞性肾细胞癌中有10例（45.45%）癌及相应癌旁组织均发生LOI;5例乳头状肾细胞癌中有2例（40%）癌及相应癌旁组织均发生IGF-ⅡLOI;2例嫌色性肾细胞癌中有1例（50%）癌及相应癌旁组织均发生IGF-ⅡLOI;1例黏液样小管状和梭形细胞癌的癌组织发生了IGF-ⅡIOI而其相应的癌旁组织IGF-Ⅱ印迹正常.并且在14岁到68岁各年龄段都有IGF-ⅡLOI的发生,且发生频率接近,表明IGF-ⅡLOI的发生与肾细胞癌的病理类型及年龄相关性不大.本实验涉及的30例肾细胞癌中,按照TNM分类,8例T1期肾细胞癌中有6例（75%）癌及其相应癌旁组织均发生了IGF-Ⅱ的LOI,1例（12.5%）在癌组织中发生IGF-ⅡLOI,但其相应癌旁组织中IGF-Ⅱ印迹正常.结论：IGF-ⅡLOI可能是肾细胞癌的早期发生事件,可能对其早期发生有重要作用.

英文摘要：

Objective： To investigate the imprinting status of IGF- Ⅱ gene in renal cell carcinoma and its relationship with the progression of renal cell carcinoma. Methods： Renal cell carcinoma tissues and matched histologically normal kidney tissues of 30 patients were collected. Based on a polymorphism ofApa I site in exon 9 of IGF- Ⅱ, polymerase chain reaction （PCR）-restriction fragment length polymorphism （RFLP） analysis was used to select the heterozygous allele. Loss of imprinting of IGF- Ⅱ was further examined with RT-PCR-PFLP technique. Results： There were 19 renal cell carcinoma （：ases with heterozygous IGF-Ⅱ gene （63.3%, 19/30）. And in these 19 case, 13 cases had loss of imprinting of IGF- Ⅱ gene （68.4%, 13/19）in carcinoma tissues and matched histologically normal kidney tissues, and 1 case had loss of imprinting of IGF- Ⅱ gene only in carcinoma tissues （5.3%, 1/19）. Of the 22 cases of clear cell renal cell carcinoma, 10 cases had loss of imprinting of IGF- Ⅱ gene in carcinoma tissues and matched histologically normal kidney tissues （45.45%, 10/22）. Of the 5 cases of papillary renal cell carcinoma, 2 cases had loss of imprinting of IGF- Ⅱ gene in carcinoma tissues and matched histologically normal kidney tissues （40%, 2/5）. Of the 2 cases of chromophobe renal cell carcinoma, 1 case had loss of imprinting of IGF-Ⅱ gene in carcinoma tissues and matched histologically normal kidney tissues. The 1 case of mucinors tubular and spindle cell carcinoma had loss of imprinting of IGF- Ⅱ gene only in carcinoma tissues. Of the 8 T1 stage heterozygous renal cell carcinoma cases, 6 cases （75%） had loss of imprinting of IGF- Ⅱ gene in carcinoma tissues and matched histologically normal kidney tissues, and 1 case （12.5%） had loss of imprinting of IGF- Ⅱ gene only in carcinoma tissues. Cenclusion： Loss of imprinting of IGF- Ⅱ gene may be an early event of renal cell carcinoma and may play an important role in the development of renal cell carcinoma.