中文摘要：

目的体外细胞培养观察利胆药物熊脱氧胆酸（ursodeoxycholic acid，UDCA）及其衍生物牛磺熊脱氧胆酸（tauroursdeoxycholic acid，TUDCA）或利福平（rifampicin，Rif）对多耐药相关蛋白3（multidrug resistance—associate protein3，MRP3）及其核受体FTF（fetoprotein transcription factor）表达的影响。方法用UDCA及其衍生物TUDCA或Rif分别刺激培养的肝癌细胞HepG2后，抽提HepG2的总RNA与细胞总膜蛋白、核蛋白，分别用Quantitative real—time PCR和Western blot检测MRP3和FTFmRNA与蛋白水平表达的变化。结果UDCA及其衍生物TUDCA与Rif均可诱导HepG2细胞内核受体FrF表达增高，同时MRP3mRNA和蛋白水平表达也相应增高。结论UDCA及其衍生物TUDCA和Rif刺激肝癌细胞HepG2细胞膜转运蛋白MRP3表达上调可能与核受体FTF途径相关。

英文摘要：

Objective To observe the effect of ursodeoxycholic acid （UDCA）, and its derivative, tauroursdeoxyeholie acid （TUDCA）, or rifampiein （Rif） on the expressions of membrane transporter, muhidrug resistance-associate protein 3 （ MRP3 ）, and its nuclear receptor, fetoprotein transcription factor （FTF） in HepG2 cells. Methods Both total RNA and total membrane or nuclear proteins were extracted from HepG2 cells after 48 h treatment with UDCA, TUDCA or Rif （100, 100 or 10 μmol/L）. The mRNA and protein expression levels of MRP3 and FTF were determined by quantitative real-time PCR and Western blot analysis. Results Both FTF and MRP3 expression was significantly induced either at mRNA or protein level by UDCA, TUDCA or Rif in HepG2 cells. Conclusion The up-regulation of hepatic MRP3 expression induced by UDCA, TUDCA or Rif may be associated with FTF pathway.