中文摘要：

目的：研究高尿酸条件下尿酸盐阴离子转运体1（Urate anion exchanger 1,URAT1）、葡萄糖转运体9（glucose transporter 9,GLUT9）、有机阴离子转运体10（Organic Anion Transporter 10,OAT10）基因表达的动态变化。方法：（1）研究不同剂量尿酸对小鼠血尿酸水平的影响：遵循随机化原则将50只雄性昆明种小鼠分为5组（每组10只）：分别为正常组、尿酸每天100、200、300、400mg/kg剂量组。每天2次腹腔注射尿酸磷酸盐溶液,共造模14天,确定最佳剂量。（2）确定最佳剂量后,小鼠腹腔注射最佳剂量尿酸每天300 mg/kg,复制高尿酸血症模型。在给予尿酸1、3、7、14天处死小鼠,用磷钨酸法测定小鼠血清尿酸水平和肝尿酸含量,用RT-PCR检测小鼠肾脏尿酸盐重吸收转运体URAT1、GLUT9、OAT10基因表达水平。结果：（1）腹腔注射尿酸每天300 mg/kg及400mg/kg能有效升高小鼠血尿酸水平（P〈0.01）,但400 mg/kg组小鼠死亡率高。而尿酸100、200 mg/kg组与正常组相比无统计学差异,300 mg/kg为最佳剂量;（2）与正常组相比,模型组血尿酸水平在造模1、3、7、14天均升高（P〈0.05）,造模成功;与正常组相比,模型组肝匀浆尿酸含量在造模1天显著升高（P〈0.01）;而造模3天、7天及14天均无统计学差异;与正常对照组相比,模型组小鼠肾脏GLUT9基因表达水平在1、3、14天均增加（P〈0.05）;与正常对照组相比,模型组小鼠肾脏URAT1和OAT10基因表达水平在各时间点均无显著变化（P〉0.05）。结论：在尿酸诱导的高尿酸血症小鼠模型上,模型组GLUT9基因在给予尿酸1、3、14天后表达均上调,但URAT1、OAT10的基因表达均无显著变化。

英文摘要：

Objective： To investigate timeliness（ chronergy） of gene expression of three renal urate transporters associated with reabsorption in kidney,including urate anion exchanger 1（ URAT1）,glucose transporter 9（ GLUT9）,and organic anion transporter 10（ OAT10） in hyperuricemic mice. Methods： 1 To get an insight into the effects of different doses of uric acid on serum uric acid in mice,50 Kunming male mice were randomly divided into five groups with10 mice for each. Long-term hyperuricemic mice model groups were copied by using intraperitoneal injection of uric acid at a dose of 100,200,300 and 400 mg / kg. d twice daily for 14 d. 2 After optimal dose is determined,intraperitoneal injection of uric acid at the dose of 300 mg / kg. d was used to establish the hyperuricemic mice model. Samples were harvested and phosphotungstic acid method was used to assay the uric acid level in serum and hepatic homogenate after 1,3,7,and 14 d＇s injection of uric acid.Tissues were quick frozen in liquid nitrogen and prepared for investigating the gene expression of GLUT9,OAT10 and URAT1 in kidney of hyperuricemic mice using RT-PCR. Results： Compared with normal group,serum uric acid level of model group was much higher（ P〈0. 01）at the dose of 300 mg / kg,400 mg / kg daily,but the mouse mortality rate were high at the dose of 400 mg / kg daily. compared with normal group,there was no difference of 100 and 200 mg / kg（ P〉0. 05）,the dose of 300 mg / kg daily was the optimal. Compared with normal group,serum uric acid level of model group was much higher（ P〈0. 05） at 1,3,7 and 14 d post model copy,which suggested the model was successfully established. Compared with the normal group,hepatic homogenate uric acid levels of model group was significantly increased post 1 d model copy（ P〈0. 01）,while there was no difference post 3,7 and 14 d model copy（ P〉0. 05）. Expression mRNA of GLUT9 was differently increased post 1,3 and 14 d post model copy（ P〈0. 05）. Whereas there was no any diff