中文摘要：

【目的】化学感受蛋白（chemosensory proteins,CSPs）在昆虫化学信号的感受识别和生长发育调节等生理过程具有重要作用。本研究旨在探索CSP16在家蚕Bombyx mori中的功能。【方法】利用RT-q PCR分析csp16在家蚕不同发育时期和不同组织的表达特征,用原核表达系统对家蚕CSP16进行表达纯化,并通过荧光结合实验检测该蛋白与不同配体化合物的结合特性。【结果】RT-q PCR结果表明,csp16在家蚕1-5龄幼虫中呈规律性表达,各龄期眠蚕中表达量最高,5龄第3天幼虫中主要表达于头、表皮、精巢和卵巢等。蜕皮激素（20E）处理后csp16在不同龄期幼虫和5龄幼虫不同组织中的表达量上调。纯化后CSP16的荧光结合实验表明,CSP16与醇类、酯类、醛类、酚类和苯环类等化合物的亲和力都较弱。【结论】csp16在家蚕不同龄期幼虫眠蚕中表达量最高,蜕皮激素使csp16在家蚕取食幼虫中的表达量出现上调,提示其可能参与家蚕幼虫的蜕皮过程。

英文摘要：

【Aim】Chemosensory proteins（ CSPs） play important roles in reception and recognition of chemical signal,and regulation of insect development. This study aims to explore the function of CSP16 in the silkworm,Bombyx mori. 【Methods 】 Real-time quantitative PCR（ RT-q PCR） was used to investigate the expression patterns of csp16 gene in different developmental stages and different tissues of the 5th instar larvae of B. mori. Prokaryotic expression system was used to express and purify CSP16.The binding properties of CSP16 were analyzed by fluorescence binding assay. 【Results】The RT-q PCR results showed that csp16 was regularly expressed in the 1st- 5th instar larvae,and highly expressed in molting larvae of each instar. In day-3 5th instar larvae,csp16 was mainly expressed in head,epidermis,testis and ovary. The expression level of csp16 was up-regulated in larvae of different instars and different tissues of the 5th instar larvae after treatment with 20-hydroxyecdysone. Purified CSP16 showed weak affinity to different compounds including alcohols, esters, aldehydes, phenols and benzene.【Conclusion】Csp16 is highly expressed in molting larvae of each instar of B. mori,and the expression level is up-regulated by 20-hydroxyecdysone in larvae during feeding,suggesting that CSP16 may be involved in molting process of silkworm larvae.