中文摘要：

本研究旨在构建一种能快速血管化的人工真皮替代物。用冻干法制备了胶原／壳聚糖多孔支架，并对其进行肝素化，观察此支架的结构特征、亲水性、体外降解性和组织相容性，同时将血管生成素引入到此支架，对复合有血管生成素的肝素化支架的体内血管化进行了初步研究。结果表明，肝素化胶原／壳聚糖多孔支架具有合适的三维多孔结构、良好的吸水性和较理想的酶解稳定性，体内实验表明，此支架具有良好的组织相容性，血管生成素可加快支架的血管化。

英文摘要：

To develop an artificial dermal substitute capable of receiving rapid angiogenesis. Collagen/chitosan porous scaffold was fabricated by freeze-drying method, and heparinized using N-（ 3-dimethylaminopropyl）-N＇ ethylcarbodiimide （EDC） and N-hydroxysuccinimide （NHS）. The morphology, hydrophilicity, in vitro biodegradation and tissue compatibility of the scaffold were investigated. After combined with angiogenin, the in vivo angiogenesis of the scaffold was studied as well. The results showed that with a suitable three-dimensional porous structure, the heparinized collagen/chitosan porous scaffold possessed good hydrophilicity and stability for collagenase degradation. In vivo study also demonstrated that the heparinized scaffold had good tissue compatibility, and its angiogenesis could be enhanced by angiogenin.