中文摘要：

目的：研究腺病毒介导的肿瘤生长抑制因子4（inhibitor of growth family 4，ING4）联合放疗对肺腺癌SPC．A1细胞移植瘤生长的抑制作用。方法：制备SPC—A1细胞荷瘤小鼠模型，随机分为PBS组、Ad组、Ad．ING4组、放疗组、Ad．ING4＋放疗组。测量各组荷瘤小鼠移植瘤的体积变化，治疗15d后摘取瘤块，称质量并计算抑瘤率；H—E染色观察瘤体组织细胞的形态学变化，免疫组化法检测瘤体组织中Bax、caspase．3、Bcl-2、VEGF等因子的表达。结果：治疗后第15天SPC．A1移植瘤体积：Ad—ING4组为（1136．03±151．58）mm3、单纯放疗组为（1035．67±86．27）mm3、Ad—ING4＋放疗组为（743．84-4-109．06）mm3，联合组可有效抑制肿瘤生长（P〈0．01）；此外，联合组抑瘤率也显著高于单纯Ad．ING4组或放疗组（69．62％粥33．17％、35．41％，P〈0．01），呈现放疗增敏协同作用（Q=1．22）。免疫组化结果显示，Ad—ING4及其联合放疗组能明显上调Bax、caspase-3等蛋白的表达，下调Bcl-2、VEGF等蛋白的表达，且Ad—ING4＋放疗组对这些蛋白表达的调节作用强于Ad．ING4组、单纯放疗组（P〈0．01）。结论：Ad—ING4联合放疗可有效抑制肺腺癌SPC—A1细胞移植瘤的生长。

英文摘要：

Objective： To study the anticancer effect of adenovirus-mediated inhibitor of growth family 4 （Ad-ING4） combined with radiotherapy on SPC-A1 transplanted lung adenocarcinoma. Methods： SPC-A1 tumor-bearing mice were established and anticancer experiment was conducted on these mice after being randomly divided into 5 groups： PBS group, Ad group, Ad-ING4 group, radiotherapy group and Ad-ING4 combined with radiotherapy group. Tumor growth was recorded by volume. All tumors were taken out and weighed at 15 days after treatment. The morphology of lung adenocar- cinoma SPC-A1 cells was observed by H-E staining. The expressions of Bax, easpase-3, Bel-2, and VEGF were detected by immunohistochemisty. Results： The tumor volume of the Ad-ING4 combined with radiotherapy group was much smaller than that of the Ad-ING4 group and the single radiotherapy group （ [ 743.84 ± 109.06 ] mm3 vs [ 1136.03 ±151.58 ] mm3, [ 1035.67 ± 86.27 ] mm3, P 〈 0.01 ）. The anticancer rate of the Ad-ING4 combined with radiotherapy group was also much better than that of the Ad-ING4 group and the single radiotherapy group （69.62% vs 33.17% vs 35.41% , P 〈 0. 01 ）, which showed an enhanced radiosensitivity and a synergetie effect （ Q = 1.22 ）. Immunohistochemistry results showed that Ad-ING4 combined with radiotherapy could obviously up-regulate the exoressions of Bax, casoase-3 and down-regulate the expressions of Bcl-2 and VEGF. And the effect of the combined group was better than the single Ad-ING4 or radiotherapy group （P 〈 0.01 ）. Conclusion： Ad-ING4 combined with radiotherapy can effectively inhibit the growth of SPC-A1 cell-transplanted lung adenocarcinoma.