中文摘要：

目的：探讨金丹活性部位对心肌缺血再灌注损伤大鼠c—fosmRNA表达的影响。方法：将金丹活性部位对心肌缺血再灌注损伤大鼠进行干预，应用RT．PCR法检测大鼠心肌c—fosmRNA表达的变化。结果：与模型组比较，各治疗组c—losmRNA的表达均有明显下降，水洗脱组c-fosmRNA的表达降低明显（P〈0．05）；预处理组与60％乙醇洗脱组C—fosmRNA的表达降低最为明显（P〈0．01）。结论：金丹60％乙醇洗脱组分与水洗脱组分可能通过下调c—fosmRNA表达而发挥对心肌缺血再灌注损伤的保护作用。

英文摘要：

Objective： To explore the effect of the active sites from Hypericum attenuatum Choisy and Salvia miltiorrhiza on the expression of c-fos mRNA in myocardial ischemia-reperfusion injury rats. Methods： We intervene on myocardial ischemia- reperfusion injury rats by active sites from Hypericum attenuatum Choisy and Salvia miltiorrhiza, and observe the expression of c-fos mRNA of myocardial infarction of rats by RT-PCR methods. Results： Compared with model group, the expression of c-fos mRNA of treatment groups all decreased. The water elution group could significantly reduce the c-los mRNA expression, and the difference was significant （P〈0.05）. The 60% ethanol elution and pre-treatment group could reduce the c-fos mRNA expression, and the difference was significant （P〈0.01）. Conclusion： Both the 60% ethanol elution and water elution group could significantly reduce the c-fos mRNA expression induced by myocardial ischemia-reperfusion injury, and it showed that they might play a role of protection of myocardial ischemia-reperfusion injury by reducing c-fos mRNA expression.