我们分析了作文并且从白杨锯蝇学习了分泌物的简历活动, Stauronematus compressicornis (赘言作家) 。一个叶三明治方法被使用测试分泌物的简历活动。Coomassie 灿烂的蓝、煤气的层析(GC ) 和另外的方法被用来分析并且执行蛋白质试金,氨基酸分析,丰满的酸和分泌物的胆固醇察觉。结果证明分泌物有重要的反在粟蚕(Pseudaletia separata 沃克) 的第 4 中间形态幼虫上喂效果。溶解在 0.27 摩尔· L −1 HCl, 0.1 摩尔· L −1 NaOH 和 0.05 摩尔· L −1 Na 2 HPO 4 独立,分泌物有反喂从 22.7% ~ 49.6%的率。同时,对待分泌物的昆虫的重量是比控制的那些显著地低的。生物化学的察觉证明在分泌物包含的蛋白质由 16 氨基酸组成。分泌物也包含了胆固醇和丰满的酸的小数量,它包括了 lauric 酸,肉豆蔻酸酸, myristoleic 酸,十七碳的酸和油的酸。
We analyzed the composition and studied the bioactivity of secretion from the poplar sawfly, Stauronematus compressicornis (Fabricius). A leaf-sandwich method was applied to test the bioactivity of the secretion. Coomassie brilliant blue, gas chromatography (GC) and other methods were used to analyze and carry out protein assays, amino acid analyses, fatty acid and cholesterol detection of the secretion. Results show that the secretion had a significant antifeeding effect on 4th instar larvae of the armyworm (Pseudaletia separata Walker). Dissolved in 0.27 mol.L^-1 HCl, 0.1 mol.L^-1 NaOH and 0.05 mol.L^-1 Na2HPO4 separately, the secretions had antifeeding rates ranging from 22.7% to 49.6%. Meanwhile, the weights of the secretion-treated insects were significantly lower than those of the control. Biochemical detection showed that the proteins contained in the secretion were composed of 16 amino acids. The secretion also contained small amounts of cholesterol and fatty acids, which included lauric acid, myristic acid, myristoleic acid, seventeen carbonic acids and oleic acid.