中文摘要：

动粒-微管的正确结合和纺锤体信号通路调控着染色体的正确分离,保证了染色体的稳定性。Spc24是参与动粒-微管相互作用的Ndc80复合体的成分之一。本文采用生物信息学手段来分析在真菌和脊椎动物共13种生物中Spc24基因的分子进化情况。分析表明,Spc24基因的开放阅读框几乎都在590-610 bp之间,编码序列比较保守。大部分生物有以TGA作为终止密码子的倾向。Spc24基因外显子有从少到多的过程,最后趋于有5个外显子。哺乳动物间氨基酸序列高度同源,最后的90个氨基酸是Spc24最为保守的区域。以上研究结果提示Spc24是一个较为保守的基因,它的稳定表达可能对染色体的稳定性起着关键作用。

英文摘要：

The right combination of kinetochore- microtubule and spindle signaling pathways regulating the correct separation of chromosome,guarantee the stability of chromosome. Spc24 is one of the components of the Ndc80 complex which is involved in the interaction of the kinetochore and microtubule. In this paper,the molecular evolution of Spc24 gene in 13 species of fungi and vertebrates is analyzed by means of bioinformatics. The analysis shows that the open reading frame of Spc24 gene is almost always between590- 610 bp,and the sequence of encoding is relatively conservative. Most creatures have a tendency to TGA as a stop codon. The exon of Spc24 gene has a process from less to more,finally tend to have five exons. The amino acid sequence is highly homologous between mammals,and the last 90 amino acids are the most conserved regions of Spc24. These results suggest that Spc24 is a more conservative gene,and its stable expression may play a key role in the stability of chromosome.