中文摘要：

为了从发酵液中分离纯化出α-酮戊二酸,作者利用活性炭对发酵液进行脱色后,采用离子交换法对α-酮戊二酸的纯化工艺进行了研究.结果表明,活性炭用量1.5 g/L、pH 3.0、脱色时间1h、脱色温度50℃的条件下能取得最佳脱色效果,脱色率达89.2％.分析了6种阴离子交换树脂对α-酮戊二酸的吸附容量,确定了吸附性能显著的D301大孔弱碱型树脂.在固定床上上样条件优化表明,当发酵液中α-酮戊二酸质量浓度为20 g/L、上样流速为2.67 BV/h、径高比为1∶12时,上样吸附效果最佳.采取阶段洗脱工艺：第一阶段用0.01 mol/L的NaC1和0.01 mol/L的HC1混合溶液进行洗脱,第二阶段采用0.3 mol/L的HCl溶液在6 BV/h的流速下进行洗脱.验证结果表明,在此洗脱工艺下,收集液中α-酮戊二酸与丙酮酸的质量比由初始发酵液中的1.5∶1提高为10∶1时,α-酮戊二酸收率为93.2％.因此,采用离子交换法可有效的分离纯化发酵液中的α-酮戊二酸.

英文摘要：

In order to purify α-ketoglutarate acid from fermentation broth,the purification technology of α-ketoglutarate acid by ion-exchange method was studied after decolored by activated carbon.The results showed as follows.Firstly,the decolorization ratio reached 89.2% under the following optimal conditions：carbon dosage 1.5 g/L,pH 3,bleaching time 1 h,bleaching temperature 50 ℃.Subsequently,the process of ion-exchange was studied and among 6 different resins D301 weakly-based anion resin was the optimal one for α-ketoglutaric acid abstraction by comparison of the adsorption capacity.And the optimal absorption efficiency was achieved when the α-ketoglutarate acid concentration in the fermentation broth was 20 g/L,the sample flow rate was 2.67 BV/h and the diameter to height ratio was 1∶12.Finally,a process of stepwise elution was investigated.In the first step,O.O1 mol/L NaCl and 0.01 mol/L HCl mixed solution was used as eluent and in the second step,0.3 mol/L HCl was used as eluent and the flow rate was 6 BV/h.With the elution strategy above,the final yield of α-ketoglutaric acid reached 93.2% and an excellent purification result was achieved with the increased mass ratio of α-ketoglutaric acid to pyruvic acid from 1.5∶1 to 10∶1.Therefore,ion-exchange method was valid to purify α-ketoglutaric acid from fermatation borth.