欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
未配对电子与蛋白质分子自旋核的作用能提供丰富的长程结构信息，这些顺磁信息通常可用顺磁弛豫增强、赝接触位移和残余偶极耦合描述，其中赝接触位移包含生物大分子内重要的距离和角度信息。稀土离子具有相似的配位化学性质和不同的顺磁物理特性，而大多稀土离子具有磁各向异性，在与大分子作用过程中会产生赝接触位移。由于大多数蛋白质没有顺磁中心，获得这些顺磁信息需要通过定点选择标记蛋白质来实现。该文旨在对近年来蛋白质顺磁标记的方法和进展进行介绍，在顺磁标记基础上阐述赝接触位移在结构生物学中的应用。
英文摘要：
The interactions between unpaired electrons and nuclear spins in proteins, usually represented in paramagnetic relaxation enhancement （PRE）, pseudocontact shift （PCS） and residual dipolar couplings （RDC）, provide rich sources of structural restraints. These paramagnetic effects are valuable in structure determination of protein and protein-ligand complex as well as in elucidation of protein dynamics. Because most proteins do not have paramagnetic centers, generation of paramagnetic restraints usually relies on site-specific labeling of proteins with paramagnetic ions. In this paper, efforts towards paramagnetic labeling of proteins, especially with lanthanide ions, are summarized, and PCS, as a powerful NMR spectroscopic tool for structural biology, is discussed.
同期刊论文项目
同项目期刊论文
Thiol-ene reaction: a versatile tool in site-specific labelling of proteins with chemically inert ta
Small Molecules Based on Benzo[1,2-b:4,5-b ']dithiophene Unit for High-Performance Solution-Processe
Chiral bifunctional squaramide catalyzed asymmetric Michael addition of ethyl a-nitroacetate to b,g-
Asymmetric Organocatalytic Cascade Michael/Hemiketalization/Retro-Aldol Reaction of 2-[(E)-2-Nitrovi
Phosphine-Catalyzed Annulations between Modified Allylic Derivatives and Polar Dienes and Substituen
Metal-Free, Stereospecific Bis-Silylation of Functionalized Alkynes with NHC-Supported Silylaminosil
Controllable and efficient oxidation of thioether by 2-iodoxybenzoic acid (IBX) in water: semisynthe
D and E Rings May Not Be Indispensable for Antofine: Discovery ofPhenanthrene and Alkylamine Chain C
Mild and highly efficient metal-free oxidative acyanation of N-acyl/sulfonyl tetrahydroisoquinolines
Enantioselective Synthesis of trans-Dihydrobenzofurans via Primary Amine-Thiourea Organocatalyzed In
Cascade Michael–Intramolecular Transesterification–Elimination Reaction of 3,4-Dihydro-3-nitrochrome
A Mild and Efficient Direct alpha-Amination of beta-Dicarbonyl Compounds Using Iodosobenzene and p-T
N-Heterocyclic Carbene Organocatalysts for Dehydrogenative Coupling of Silanes and Hydroxyl Compound
Recyclable Hypervalent Iodine(III) Reagent Iodosodilactone as an Efficient Coupling Reagent for Dire
Direct asymmetric hydrogenation of a-keto acids by using the highly efficient chiral spiro iridium c
Catalytic Asymmetric Hydrogenation of alpha-Arylcyclohexanones and Total Synthesis of (-)-alpha-Lyco
The First Enantioselective Approach to 13a-Methyl-14-hydroxyphenanthroindolizidine Alkaloids - Synth
Design, Synthesis, and Anti-tobacco Mosaic Virus (TMV) Activity of Phenanthroindolizidines and Their
Design, Synthesis, Anti-Tobacco Mosaic Virus (TMV) Activity, and SARs of 7-Methoxycryptopleurine Der
Enantioselective Organocatalytic Domino Michael/Aldol Reactions: An Efficient Procedure for the Ster
Phosphane-Catalyzed [4+1] Annulation between Nitroalkenes and MoritaBaylis-Hillman Carbonates: Facil
Phosphine-catalyzed formal vinylogous aldol reaction of gamma-methyl allenoates with aldehydes: easy
Design, synthesis, antiviral activity, and SARs of 13a-substituted phenanthroindolizidine alkaloid d
An enantioselective strategy for the total synthesis of (S)-tylophorine via catalytic asymmetric all
Sodium Nitrite-Catalyzed Aerobic Oxidative Csp2 Csp3 Coupling: Direct Construction of the 4-Aryldihy
Synthesis and Antiviral and Fungicidal Activity Evaluation of beta-Carboline, Dihydro-beta-carboline
Rhodium(III)-Catalyzed Alkenylation Reactions of 8-Methylquinolines with Alkynes by C(sp(3))-H Activ
First asymmetric synthesis of Silodosin through catalytic hydrogenation by using Ir-SIPHOX catalysts
Copper-Catalyzed Intramolecular Trifluoromethylation of N-Benzylacrylamides Coupled with Dearomatiza
Kinetic Assay of the Michael Addition-Like Thiol-Ene Reaction and Insight into Protein Bioconjugatio
Copper-catalyzed B-H bond insertion reaction: a highly efficient and enantioselective C-B bond formi
Stereoselective Synthesis of Highly Functionalized Nitrocyclopropanes through the Organocatalyic Mic
Stereocontrolled Construction of the 3,4-Dihydrothiacarbazol-2(9H)-one Skeleton by Using Bifunctiona
Remote Ester Group Leads to Efficient Kinetic Resolution of Racemic Aliphatic Alcohols via Asymmetri
An in situ acidic carbon dioxide/glycol system for aerobic oxidative iodination of electron-rich aro
Phosphine-catalyzed [4+1] annulation of 1,3-(aza)dienes with maleimides: highly efficient constructi
Intramolecular oxo-Diels–Alder rearrangement in collisionally activated dissociation of protonated i
Synthesis, structures, and norbornene polymerization behavior of C(sp3), N-chelated palladacycles be
Phosphine-catalyzed [4+2] annulation and vinylogous addition reactions between 1,4-dien-3-ones and 1
A Simple and Effective Synthesis of Benzolactones and Benzolactams by Noncatalytic Benzylic Oxidatio
Catalytic fixation of CO2 to cyclic carbonates by phosphonium chlorides immobilized on fluorous poly
Novel thiophosphonodiamides as efficient hydrogen bond donor catalysts in tandem Michael additionecy
Reduction of sulfoxides and pyridine-N-oxides over iron powder with water as hydrogen source promote
Highly Efficient SO2 Absorption and Its Subsequent Utilization by Weak Base/Polyethylene Glycol Bina
Divergent Amine-Catalyzed [4+2] Annulation of Morita-Baylis-Hillman Allylic Acetates with Electron-D
Discovery and SARs of Trans-3-Aryl Acrylic Acids and Their Analogs as Novel Anti- Tobacco Mosaic Vir
4-(N,N-Dimethylamino)pyridine Hydrochloride as a Recyclable Catalyst for Acylation of Inert Alcohols
Diastereoselective Synthesis of Functionalized Spirocyclopropyl Oxindoles via P(NMe2)3？Mediated Redu
Use of graphene as a matrix to minimize reduction in the process of matrix-assisted laser desorption
Cascade Electrophilic Iodocyclization: Efficient Preparation of 4-Iodomethyl Substituted Tetrahydro-
Bioinspired Construction of a Spirocyclohexadienone Moiety via Sodium Nitrite Catalyzed Aerobic Intr
A new binol-derived chiral bifunctional phosphine organocatalyst: preparation and application in asy
Selective 15N-labeling of the side-chain amide groups of asparagine and glutamine for applications i
Synthesis, Structures, and Norbornene Polymerization Behavior of o？Aryloxide-Substituted NHC-Ligated
A Convergent and Stereocontrolled Cycloaddition Strategy toward Eudesmane Sesquiterpenoid: Total Syn
Synthesis of Oxazolidin-2-ones and Imidazolidin-2-ones Directly from 1,3-Diols or 3-Amino Alcohols u
Ruthenium Ring-Opening Metathesis Polymerization Catalysts Bearing o- Aryloxide-N-Heterocyclic Carbe
Highly efficient hydrogenation of biomass-derived levulinic acid to gamma-valerolactone catalyzed by
Rh(III)-catalyzed synthesis of 1-aminoindole derivatives from 2-acetyl-1-arylhydrazines and diazo co
Design, synthesis, anti-TMV, fungicidal, and insecticidal activity evaluation of 1,2,3,4-tetrahydro-
Synthesis of Optically Active 2H-ThiopyranoACHTUNGTRENUNG[2,3-b]quinolines with Three Contiguous Ste
Highly Chemoselective Rauhut-Currier Reaction between Maleimides and Enones and Dual Phosphine-Media
Bifunctional Squaramide-Catalyzed One-Pot Sequential Michael Addition/Dearomative Bromination: Conve
Design of task-specific ionic liquids for catalytic conversion of CO2 with aziridines under mild con
Collective Asymmetric Synthesis of (-)-Antofine, (-)-Cryptopleurine, (-)-Tylophorine, and (-)-Tylocr
Regioselective Ortho Olefination of Aryl Sulfonamide via Rhodium- Catalyzed Direct C？H Bond Activati
Lewis Acid Catalyzed Formal Intramolecular [3+2] Cross-Cycloaddition of Cyclopropane 1,1-Diesters wi
An Enantioselective Strategy for the Synthesis of (S)-Tylophorine via One-Pot Intramolecular Schmidt
Equimolar Carbon Absorption by Potassium Phthalimide and In Situ Catalytic Conversion Under Mild Con
Design, Synthesis, and Antiviral, Fungicidal, and Insecticidal Activities of Tetrahydro-β-carboline-
期刊信息
位置：