为研究甲氨基阿维菌素苯甲酸盐对西花蓟马的控制作用,室内采用浸叶法测定了其对西花蓟马成虫和二龄若虫的毒力。同时,测定了甲维盐LC50短时胁迫不同时间后,西花蓟马可溶性蛋白质、可溶性糖及脂肪三种能源物质含量的变化;其致死后,计算了昆虫对各能源物质的利用率,以期从生理生化角度探讨其作用机制。结果表明,甲维盐对西花蓟马成虫和若虫的LC50分别为1.11和0.62 mg/L,在其LC_(50)短时胁迫过程中,西花蓟马成虫体内的能源物质含量均伴有显著的升高及降低,但若虫并无显著变化;其LC_(50)致死下,西花蓟马对三种能源物质的利用率存在显著差异,成虫为可溶性糖(74.12%)〉可溶性蛋白质(58.10%)〉脂肪(42.13%),若虫为可溶性糖(64.01%)〉可溶性蛋白质(48.12%)〉脂肪(36.48%),且无论何种能源物质,西花蓟马成虫的利用率均显著高于若虫。
In order to examine the effect of emamectin benzoate to Frankliniella occidentalis,the toxicity of this insecticide to the adult and 2nd instar of F. occidentalis were studied by dip method in this paper.In addition,the content of soluble protein,soluble sugar and lipid in F. occidentalis were also tested after these insects were exposed to medial lethal doses of emamectin benzoate for the following exposure times:2,4,6,and 8 h. Furthermore the utilization rate of the energy source in F. occidentalis was calculated after the insects were exposed to emamectin benzoate until dead at the medial lethal doses. The results showed that emamectin benzoate had different toxicity to the adult and 2nd instar of F. occidentalis,and the medial lethal doses were 1. 11 and 0. 62 mg / L,respectively. With the extension of exposure time at medial lethal doses of emamectin benzoate,there was significant a increase or decrease on the content of soluble protein,soluble sugar and lipid in F. occidentalis adult,but no significant variation on their content in F. occidentalis nymph. When the F. occidentalis was dead under the medial lethal doses expo-sure,there was significant difference in the utilization rate of the three kinds of energy sources in F. occidentalis, the utilization was soluble sugar( 74. 12%) soluble protein( 58. 10%) lipid( 42. 13%) in adult,significantly higher than that in nymph at any kind of the three energy sources,and the utilization was soluble sugar( 64. 01%) soluble protein( 48. 12%) lipid( 36. 48%) in nymph. According to the results,we could conclude that the different variation and utilization of energy source between F. occidentalis adult and nymph under the exposure of emamectin benzoate,may have some relationship to their different tolerance to this insecticide.