中文摘要：

目的探讨低氧条件下小鼠皮肤成纤维细胞内低氧调节关键转录因子HIF-1、多潜能转录因子Oct4及表观遗传调控因子Uhrf1的表达,为低氧微环境影响细胞重编程效率的机制研究奠定基础。方法将体外培养的小鼠皮肤成纤维细胞分为低氧（2%O_2＋5%CO_2＋93%N_2）6h、12h、24h、48h组和常氧（20%O_2＋5%CO_2）对照组。用免疫荧光化学方法和RT-PCR检测小鼠皮肤成纤维细胞中HIF-1,Oct4和Uhrf1的表达,在蛋白和m RNA水平对其进行分析。结果成纤维细胞内HIF-1、Oct4、Uhrf1蛋白的阳性表达率随着低氧时间的延长逐渐增多,常氧组均未见表达。低氧6h、12h、24h组m RNA表达量均比常氧组高,但差异不显著（〉0.05）;而低氧48h组三个基因m RNA表达明显高于常氧组（〈0.01）。结论低氧微环境HIF-1,Oct4和Uhrf1在成纤维细胞内表达水平上调,提示它们在其中发挥了重要作用。

英文摘要：

Objective To investigate the expression of hypoxic key transcription factor, pluripotent transcription factor Oct4 and the epigenetic regulator Uhrf1 in skin fibroblast cells of mice under hypoxic microenvironment. Methods The mice skin fibroblasts were cultured in vitro, and divided into hypoxia（2%O ＋5%CO ＋93%N） experiment 2 2 2component group（6h, 12 h, 24 h, 48h）, normoxia（20%O ＋5%CO） as control group. Immunofluorescence and RT-PCR 2 2technique were used to detect the expression of HIF-1, Oct4 and Uhrf1 protein and m RNA respectively. Results With the extention of hypoxia time, the positive expression rates of HIF-1, Oct4 and Uhrf1proteins were increased, but negative for normoxia group. The expression levels of H I F- 1, O c t 4 a n d U h r f 1 mR NA were increased in hypoxia 6h, 12 h, 24 h groups, but with no significant difference（ P〉0.05）, while obviously higher in hypoxia 48 h group than in normoxia（ P〈 0.01）. Conclusion The upregulation of the expression levels of HIF-1, Oct4 and Uhrf1 in hypoxic microenvironment indicates that they might play an important role in the process.