胰岛素样生长因子-I是调节动物生命活动重要的多肽生长因子之一,它对动物的生长发育及代谢有重要的调控作用。本研究根据GenBank中发表的鸡胰岛素样生长因子I基因(IGF—I)的序列针对5’调控区的TrulI位点设计1对引物。采用PCR—RFLP技术检测边鸡、京海黄鸡、尤溪麻鸡和AA鸡TrulI位点的多态性,并试图探索TrulI位点的多态性和边鸡经济性状的关系。结果表明:边鸡、京海黄鸡和尤溪麻鸡TrulI位点存在多态性,而AA鸡不存在多态性;在京海黄鸡和尤溪麻鸡中形成AA、AB和BB3种基因型,在边鸡中仪存在AA和AB基因型,基因型分布在4个鸡品种中并不一致。该位点能否作为边鸡经济性状的一个遗传标记有待进一步研究。
The insulin-like growth factor I (IGF-I) is one of the important polypeptide growth factor in regulating animal life activities, it is important regulator of growth and metabolism to animals. One pair of primers were designed to amplify the 5' regulator region of IGF-I gene, based on the chicken DNA sequence of the ICF-I gene in the GenBank. PCR-RFLP was used to deeteet the polymorphsims of the Trul I locus in tile Bian, Jinghai, Youxi and AA chickens, and try to explore the relationship between the polymorphisms of Trul I loci and economic traits of Bian chicken. The results showed that the Bian, Jinghai and Youxi chickens displayed polymorphisms, and AA chicken was monomorphic. AA, AB and BB genotypes were formed in the Jinghai and Youxi chickens. Only two genotypes (AA and BB) were detected in the Bian chicken. The genotypes distribution were not consistent in the four chicken Breeds. Whether the locus can be used as a genetic marker for ec'onomie trails in the Bian chickens needs further research.