中文摘要：

目的测试在加力状态下,层层自组装BMP-2控释涂层的缓释效果,并检测其对牙周膜干细胞（periodontal ligament stem cells,PDLSC）的成骨诱导作用。方法通过层层自组装技术在Flexcell加力六孔板上负载[（PAH/BMP-2）/PSS][COL/ALG]2 0涂层,利用ELISA检测加力状态下缓释涂层21 d内释放的BMP-2浓度;将4~6代PDLSCs种于预置BMP-2控释涂层的Bio Flex专用六孔培养板,置于FX-4000T加力系统中,加载动态张应力（10%形变牵张力,20 Hz）,各组作用时间均为1 h/次,3次/d,间隔4 h加力,分别培养3、5、7 d。对照组为非预置涂层添加BMP-2培养液（100 ng/ml）的Bio Flex板同条件下加力培养;使用用Real-Time PCR和Western印迹法分析PDLSCs在不同张应力作用前后,其表型标志Runx2,ALP和OCN在m RNA和蛋白水平的表达变化情况。结果实验结果显示,与对照组相比,实验组加力3、5、7 d后,Runx2、ALP和OCN标志物m RNA与蛋白表达差异有统计学意义（P〈0.05）。结论 [（PAH/BMP-2）PSS]/[COL/ALG]20涂层对h PDLSCs在加力状态下成骨向分化能力具有显著增强作用。

英文摘要：

Objective To assess the effect of self-assemble BMP-2 controlled-release nano-film on osteogenic differentiation of periodontal ligament Stem cells（ PDLSCs） under mechanical force.Methods Bioflex Plates were coated with self-assembly [（ PAH / BM P-2） / PSS][COL / ALG]20multi-layers modified nano-films,and then the delivery concentration of BM P-2 was detected by ELISA. The PDLSCs were cultured into six-well Bioflex Plates pre-coated with the multi-layers modified nano-films,and then placed in the Flexcell FX-4000 T Tension Plus System. PDLSC were subjected to the distinct mechanical forces（ 10% static tension force,20 Hz,1 h × 3 / d,for 3 d,5 d or7 d）. Control cells were cultured in Bio Flex plates with BM P-2（ 100 ng / ml） instead of BM P-2 nano-films. Then the expression of Runx2,ALP and OCN m RNA and protein was detected by Real-Time PCR and Western blot techniques,respectively. Results The expressions of Runx2,ALP and OCN m RNA and protein in BM P-2 nano-biofilm group were significantly higher than those in control group（ P〈0. 05）. Conclusion The BMP-2 controlled release nano-flim can significantly promote the osteoblast differentiation of h PDLSCs cultured under mechanical force.