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普安银鲫(Carassius auratus gibelio)卵黄囊期脂蛋白脂酶和肝脂酶基因的表达及葡萄糖、维生素C对其的影响
  • ISSN号:2095-9869
  • 期刊名称:《渔业科学进展》
  • 时间:0
  • 分类:S917[农业科学—水产科学]
  • 作者机构:[1]贵州省瓮安县农村工作局,瓮安550400, [2]贵州大学动物科学学院水产科学系高原山地动物遗传育种与繁殖教育部重点实验室,贵阳550025
  • 相关基金:国家自然科学基金项目(31160527)资助
中文摘要:

为了探讨普安银鲫(Carassius auratus gibelio)卵黄囊期脂蛋白脂酶(Lipoprotein lipase,LPL)和肝脂酶(Hepatic lipase,HL)的表达特点及葡萄糖和维生素C溶液分别浸泡对它们的影响,本研究采用荧光定量PCR技术检测LPL和HL基因在普安银鲫卵黄囊仔鱼发育中的表达情况及mRNA表达水平,并检测了葡萄糖、维生素C对这两种基因mRNA表达量的影响。结果显示,LPL和HL基因在普安银鲫内源营养期、混合营养期和外源营养期均有表达,且LPL和HL mRNA表达量呈上升变化。葡萄糖组LPL和HL mRNA表达量呈上升变化,维生素C组也呈上升变化。在内源营养期、混合营养期和外源营养期,葡萄糖能显著上调LPL和HL mRNA的表达量(P〈0.05);在内源营养期、混合营养期和外源营养期,维生素C能显著上调LPL mRNA的表达量,而HL mRNA的表达量在混合营养期和外源营养期显著高于对照组(P〈0.05)。研究表明,普安银鲫发育至混合营养期时,机体内脂质分解代谢增强。适宜水平的葡萄糖、维生素C能诱导LPL和HL mRNA的表达。

英文摘要:

In this study we investigated the expression patterns of lipoprotein lipase and hepatic lipase genes, as well as the effects of the glucose solution and the vitamin C solution on its regulation during the development of the yolk-sac larva of Carassius auratus gibelio. In the preliminary experiments, the membrane break time and hatching rates were recorded to determine the optimal concentrations of glucose and vitamin C. Solutions without(control group) and with glucose(optimal concentration 15 g/L glucose group) or vitamin C(optimal concentration 30 mg/L vitamin C group) were used for hatching, and the gene expression patterns of lipoprotein lipase and hepatic lipase were analyzed. Real-time PCR was applied to measure the mRNA level of lipoprotein lipase and hepatic lipase, and to test how glucose and vitamin C regulated the expression of two genes during the development of the yolk-sac larva. We found that genes of lipoprotein lipase and hepatic lipase were expressed at the endogenous nutrition stage, the mixed nutrition stage and the exogenous nutrition stage, and that the expression was increased over time. The mRNA levels of both lipoprotein lipase and hepatic lipase were up-regulated from the endogenous nutrition stage to the exogenous nutrition stage in the group treated with 15 g/L glucose(P〈0.05). Similarly they were also re-regulated from the endogenous nutrition stage to the exogenous nutrition stage in the group treated with 30 mg/L vitamin C, which caused a significant increase in the expression of lipoprotein lipase mRNA during the yolk-sac larva developmental stage. Vitamin C increased the expression of hepatic lipase mRNA during the mixed nutrition stage and the exogenous nutrition stage(P〈0.05). Our study suggested that the lipid catabolism of C. auratus gibelio increased during the mixed nutrition stage, and that appropriate concentrations of glucose(15 g/L) and vitamin C(30 mg/L) could induce the mRNA expression of lipoprotein lipase and hepatic lipase.

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期刊信息
  • 《渔业科学进展》
  • 中国科技核心期刊
  • 主管单位:中华人民共和国农业部
  • 主办单位:中国水产科学研究院黄海水产研究所 中国水产学会
  • 主编:唐启升
  • 地址:青岛市南京路106号1号楼210室
  • 邮编:266071
  • 邮箱:yykxjz@ysfri.ac.cn
  • 电话:0532-85833580
  • 国际标准刊号:ISSN:2095-9869
  • 国内统一刊号:ISSN:37-1466/S
  • 邮发代号:24-153
  • 获奖情况:
  • 2002年获全国优秀农业期刊2003年被评为中国科技...
  • 国内外数据库收录:
  • 美国剑桥科学文摘,英国动物学记录,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2011版),中国北大核心期刊(2014版)
  • 被引量:2849