采用差速离心和不连续蔗糖密度梯度离心纯化番茄液泡膜微囊,并且测定H+-ATPase对NaNO3、NaN3和Na3VO4的敏感性。结果表明,番茄液泡膜微囊主要分布在22%的蔗糖界面上。
Tonoplast vesicles were isolated with differential centrifugation and centrifugation of discontinuous sucrose density gradient.The sensitivities of H+-ATPase to NaNO3,NaN3 and Na3VO4 were determined.The results indicated that tonoplast vesicles were mainly on the sucrose interface of 22%.