中文摘要：

目的：研究人参皂苷Rb1对不可预见性温和应激（CUMS）大鼠海马-杏仁核组织微管相关蛋白-2（MAP-2）磷酸化的影响,探讨人参皂苷Rb1抗抑郁作用的可能机制。方法：选用30只成年Wistar大鼠,随机分为对照组、应激组和干预组。给予应激组大鼠CUMS应激22 d,干预组大鼠同时给予人参皂苷Rb1。采用糖水实验和旷场实验进行行为学检测,采用Western blot方法检测海马、杏仁核组织MAP-2及p MAP-2表达,Real-time PCR检测m RNA表达。结果：（1）对照组、应激组和干预组大鼠糖水偏好百分比、旷场实验得分比较,应激组大鼠明显低于对照组,干预组低于对照组,高于应激组,差异均有统计学意义（P〈0.05）。（2）应激组大鼠海马和杏仁核组织p MAP-2蛋白和m RNA表达水平明显低于对照组（P〈0.05）,干预组表达明显升高,但仍低于对照组（P〈0.05）。结论：人参皂苷Rb1抗抑郁作用可能与影响海马、杏仁核MAP-2磷酸化有关。

英文摘要：

Objective：To observe the effcet of ginsenoside Rbl on phosphorylation of microtubule- associated protein 2 （pMAP-2）in hippocampus and amygdala of depressive rats. Methods：Thirty male Wistar rats were randomly divided into control group, stress group and treatment group. The stress group rats were gave the chronic unpredicted mild stress and the treatment grous rats were gave ginsenoside Rbl. The behavior changes were examined using sucrose preference test and open-field test. The expression of pMAP-2 protein and mRNA were detected by Western blot and real-time PCR respectively. Results： （ 1 ） Compared to control rats, preferce of sucrose and score of open-field test of stress group rats were significantly lower, while the escape latency were higher （ P〈0.05 ）. These index were reversed in treatment group. （ 2 ） The expression of pMAP-2 protein and mRNA of stess group were lower than that of control group, while the index was higher after treatment with ginsenoside Rbl （ P〈0.05 ）. Conclusion ： Effect of ginsenoside Rbl antidepressant may induced by affect the phosphorylation of MAP-2 in hippocampus and amygdala.